Addgene

We narrowed to 16,283 results for: JUN

Showing: 1081 - 1100 of 16283 results

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pIND-hygro-HA-H3.3

Plasmid

#47981

Purpose

Ponasterone A-inducible expression of human HA tagged H3F3A

Depositor

Bing Zhu

Article

Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

Insert

H3F3A (H3-3A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

288 bp T to C synonymous mutation

Promoter

Minimal Heat Shock Promoter

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pVL1393-MAL-SYK

Plasmid

#157990

Purpose

Expresses SYK in insect cell (sf9)

Depositor

Byung Il Lee

Article

Lee et al Mol Cells. 2018 Jun;41(6):545-552. doi:

Insert

Spleen tyrosine kinase (SYK Human)

Use

Tags

Met-Ala-Leu and add Leu,Glu residues +His tag

Expression

Insect

Mutation

Promoter

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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EIF4G2_MIF4G_pGEX

Plasmid

#135138

Purpose

Recombinant expression of EIF4G2 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

EIF4G2 (EIF4G2 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMG059 MBP-CREB-His

Plasmid

#154076

Purpose

Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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MIGR1-CD5

Plasmid

#159329

Purpose

Over-expression of mouse CD5

Depositor

Nevil Singh

Article

Matson et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

Insert

CD5 (Cd5 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

LTR

Available since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

Plasmid

#119067

Purpose

Donor construct to amplify NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

NLS-Cas9m4-NLS-VP64-TaV-2a-Citrine-FRT-Kan-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM-T-Easy-HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

Plasmid

#119065

Purpose

Donor construct to amplify HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT cassette for BAC recombination

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

HA-NLS-dCas9-NLS-SID4x-TaV-2a-Citrine-FRT-Kan-FRT

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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N205 nLV EF-1a-ZFHD1-link-FKBP-HA <T2A> HP1aCS-Frbx2-V5-PGK-Blast

Plasmid

#44017

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Inserts

FKBP
Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Frb, HA, Hu Frb x2(WT Frb+codon Wobble Frb), V5/e…

Expression

Mutation

N-term chromo domain removed

Promoter

EF-1a

Available since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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plexm-mouse LRRC33

Plasmid

#111601

Purpose

Express mouse LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

mouse LRRC33 (Nrros Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Starts at aa 18

Promoter

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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2xNLS-NLP-cMyc LbaCas12a

Plasmid

#182123

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc LbaCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMG058 MBP-GS 634-666-His

Plasmid

#154077

Purpose

Expresses MBP-GS 634-666-His (human 33aa Glycogen Synthase peptide as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GYS1 (GYS1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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N163 nLV EF-1a-Hp1a(CS)-Frbx2(Frb+FrbWobb)-V5-PGK-Puro

Plasmid

#44195

Depositor

Jerry Crabtree

Article

Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

Insert

Cbx5 (Cbx5 Mouse)

Use

Lentiviral

Tags

Frb, Hu Frb x2(WT Frb+codon Wobble Frb), and V5/e…

Expression

Mutation

N-term chromo domain removed

Promoter

EF-1a

Available since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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HNRNPH2_3xRRM_pGEX

Plasmid

#135115

Purpose

Recombinant expression of HNRNPH2 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

HNRNPH2 (HNRNPH2 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 23, 2021

Availability

Academic Institutions and Nonprofits only

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ILF2_DZF_pGEX

Plasmid

#135162

Purpose

Recombinant expression of ILF2 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

ILF2 (ILF2 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 14, 2020

Availability

Academic Institutions and Nonprofits only

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px335-EF-Slc2a3-L

Plasmid

#122304

Purpose

Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMG023 MBP-Axin 384-518-His (miniAxin)

Plasmid

#154012

Purpose

Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

381-518 fragment

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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CNOT4_RRM-ZNF_pGEX

Plasmid

#135146

Purpose

Recombinant expression of CNOT4 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

CNOT4 (CNOT4 Human)

Use

Tags

GST-SBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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ET1-human LRRC33

Plasmid

#111603

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Use

Tags

PreScission/TEV/ProteinC/6xHIS/SBP/

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc enAspCas12a

Plasmid

#182127

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc enAspCas12a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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ArpC3-FLAG_pLib

Plasmid

#173680

Purpose

ArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC3 (ARPC3 Human)

Use

Tags

TEV cleavage site and FLAG tag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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