We narrowed to 2,475 results for: T2A

1. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

   Plasmid

   #128762

   Purpose

   Expresses DIS3L2 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   DIS3L2 sgRNA #1 (DIS3L2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

   Plasmid

   #128760

   Purpose

   Expresses PARN gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PARN sgRNA #2 (PARN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3

   Plasmid

   #128758

   Purpose

   Expresses PAPD4 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PAPD4 sgRNA #3 (TENT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

   Plasmid

   #128756

   Purpose

   Expresses PAPD4 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PAPD4 sgRNA #1 (TENT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

   Plasmid

   #128757

   Purpose

   Expresses PAPD4 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PAPD4 sgRNA #1 (TENT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-loxP-3xpA-sfGFP-tdTomato T2A Q XE

   Plasmid

   #113852

   Purpose

   Superfolder GFP and tdTomato linked by T2A (Q linker)

   Depositor

   Mario Capecchi

   Insert

   sfGFP-T2A-tdTomato
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A V XE

   Plasmid

   #113843

   Purpose

   Histone H2B-superfolder GFP and tdTomato linked by T2A (V linker)

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-T2A-tdTomato
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG-loxP-3xpA-H2BsfGFP-tdTomato - T2A Q XE

   Plasmid

   #113842

   Purpose

   Histone H2B-superfolder GFP and tdTomato linked by T2A (Q linker)

   Depositor

   Mario Capecchi

   Insert

   H2B-sfGFP-T2A-tdTomato
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124428

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Expression

   Mammalian

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-U6-DR30(SapI)-EFS-CasRx-T2A-mCherry-pA

    Plasmid

    #192481

    Purpose

    To express CasRX and a gRNA

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    CasRx
    

    Use

    AAV

    Mutation

    No

    Promoter

    U6/EFS

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

    Plasmid

    #219548

    Purpose

    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

    Insert

    NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2
    

    Expression

    Mammalian

    Mutation

    The (SAGG)x4 linker between GAL4(1-65) and the PC…

    Promoter

    CMVd1

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

    Plasmid

    #170273

    Purpose

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
    

    Expression

    Mammalian

    Mutation

    See Comments

    Promoter

    CMVd1

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124429

    Purpose

    Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(TEV cleavage seq) T2A mCherry
    

    Expression

    Mammalian

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73040

    Purpose

    Express Nluc and copGFP dual reporter in mammalian cells

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    PGK (phosphoglycerate kinase 1)

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed

    Plasmid

    #157974

    Purpose

    Template plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9

    Depositor

    Carolyn McBride

    Article

    Zhao et al Nature. 2022 May;605(7911):706-712. doi:

    Insert

    Orco
    

    Expression

    Insect

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

    Plasmid

    #73022

    Purpose

    Luminescent and fluorescent dual reporter

    Depositor

    Kazuhiro Oka

    Insert

    Nluc-P2A-copGFP-T2A-Puro
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5/FRT/TO_TOMM20-SNAP-tag2-T2A-EGFP

    Plasmid

    #226516

    Purpose

    Mamalian cell expression of SNAP-tag2 on mitochondrial surface and cytosolic EGFP

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    TOMM20-SNAP-tag2-T2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

    Plasmid

    #172982

    Purpose

    control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    control sgRNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-H13LTat-Thy1.2-P2A-GFP-T2A-Nef

    Plasmid

    #126553

    Purpose

    Replication incompetent HIV with H13L Tat and GFP-t2a-Thy1.2 reporter

    Depositor

    Jonathan Karn

    Article

    Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

    Insert

    HIV-1 vector pNL4-3
    

    Use

    Lentiviral

    Tags

    Thy1.2 and GFP

    Promoter

    HIV LTR

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-SOX2-T2A-2xNLS-tdTomato-F2A-Puro

    Plasmid

    #89991

    Purpose

    Donor template for generation of SOX2-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

    Insert

    SOX2-T2A-2xNLS-TdTomato-F2A-Puro (SOX2 Human)
    

    Use

    CRISPR

    Promoter

    none

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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