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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,121 results for: gibson assembly

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Showing: 1081 - 1100 of 1121 results

  7. KTK_095

    Plasmid
    #180524
    Purpose
    dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly
    Depositor
    Tom Ellis
    Publication
    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    180524-plasmid-map-sequence-id-359542

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  9. pDONOR 6colors

    Plasmid
    #229974
    Purpose
    bigMamAct assembled plasmid for 6colors cellular labelling
    Depositor
    Martin Pelosse
    Publication
    Pelosse et al Synthetic Biology 2025.02.13
    Insert
    mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    See Depositor Comments
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    229974-plasmid-map-sequence-id-461259

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  11. pHR Miro1 (1-590) m-sfGFP FLAG I3-01

    Plasmid
    #248233
    Purpose
    Encodes the cytosolic portion of human Miro-1 (RHOT1) C-terminally fused with monomerized sfGFP (V206R), FLAG and the self-assembling I3-01 domain.
    Depositor
    Ron Vale
    Publication
    Gladkova et al Science. 2026 Aug 6;393(6811):601-606. d
    Insert
    Miro1-590 wt -sfGFP-60mer shorter linker (RHOT1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV
    Available since
    Aug. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    248233-plasmid-map-sequence-id-493059

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  12. pmScarlet3_I3

    Plasmid
    #239342
    Purpose
    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.
    Depositor
    Torsten Wittmann
    Publication
    Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    I3-01
    Tags
    mScarlet3
    Expression
    Mammalian
    Mutation
    K129A
    Promoter
    CMV
    Available since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239342-plasmid-map-sequence-id-474359

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Showing: 1081 - 1100 of 1121 results