We narrowed to 1,944 results for: quo

1. pTDpelB-C_sfYFPTwinStrep

   Plasmid

   #45944

   Purpose

   expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Insert

   synthetic sfYFP (codon usage adapted to P.putida KT2440)
   

   Use

   Tags

   PelB export sequence and Twin-Strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   lacIq/Ptrc

   Available since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-EGFP-P2A-FusionRed

   Plasmid

   #191108

   Purpose

   Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   pAAV-CAG-EGFP-P2A-FusionRed
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   chicken β-actin promoter

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX4T3-GST-EGFP

   Plasmid

   #177136

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-D2s-L-Venus

   Plasmid

   #19966

   Depositor

   Jonathan Javitch

   Article

   Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

   Insert

   D2 short variant-monomeric Venus (DRD2 Aequorea Victoria, Human)
   

   Use

   Tags

   Flag and monomeric Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

   Plasmid

   #174892

   Purpose

   amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG in sfGFP

   Promoter

   EF1

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFEndoNA2

   Plasmid

   #174667

   Purpose

   Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

   Depositor

   Jukka Finne

   Article

   Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

   Insert

   GFP-endoNA2 fusion protein
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_hSyn_SpikeyGi2_kv2.1

   Plasmid

   #195313

   Purpose

   Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, in AAV vector under the neuronal-specific promoter hSyn

   Depositor

   Vincent Pieribone

   Article

   Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

   Insert

   SpikeyGi2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAGGS-Flex/5'USS-GFP

   Plasmid

   #197884

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

   Plasmid

   #174891

   Purpose

   amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG in sfGFP

   Promoter

   EF1

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-D2s-L-V1

    Plasmid

    #19967

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-N terminal Venus (DRD2 Aequorea Victoria, Human)
    

    Use

    Tags

    Flag and N terminal Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pDONR P4-P1R-EYFP

    Plasmid

    #48350

    Purpose

    Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    EYFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a Kozak sequence. Does not contain a sto…

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR P2R-P3-ECFP

    Plasmid

    #48353

    Purpose

    Contributes the coding sequence for ECFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.

    Depositor

    Tomas Santalucia, Anna Planas

    Article

    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

    Insert

    ECFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Contains a stop codon.

    Promoter

    none

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGWKS10

    Plasmid

    #139417

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    Clover
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. DMP1-GFPtopaz

    Plasmid

    #114270

    Purpose

    GFP labeling of mature osteoblasts and osteocytes.

    Depositor

    David Rowe

    Article

    Kalajzic et al Bone. 2004 Jul;35(1):74-82. doi: 10.1016

    Insert

    Dmp1 + GFPtopaz (Dmp1 Aequorea victoria, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dmp1

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGWKS5

    Plasmid

    #139412

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Citrine marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    Citrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. LifeAct-mAvicFP1

    Plasmid

    #129512

    Purpose

    Expresses LifeAct-mAvicFP1 in mammalian cells

    Depositor

    Nathan Shaner

    Article

    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

    Insert

    LifeAct-mAvicFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mAvicFP1 is AvicFP1 F64L + A206K

    Promoter

    CMV

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCI.neo-rPAM1-M314I

    Plasmid

    #145802

    Purpose

    expresses catalytically inactive rat PAM-1 in mammalian cells

    Depositor

    Betty Eipper, Richard Mains

    Article

    Eipper et al Biochemistry. 1995 Mar 7;34(9):2857-65.

    Insert

    rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed methionine 314 to isoleucine to inactivat…

    Promoter

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAGGS-Flex-GFP

    Plasmid

    #197883

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. p1.1-eGFP

    Plasmid

    #162738

    Purpose

    Fluorescent reporter for CHO cells expression studies

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Insert

    enhanced green fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    consensus Kozak sequence (GCCGCCATGG) added befor…

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-D2s-L-V2

    Plasmid

    #19968

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-C terminal Venus (DRD2 Aequorea Victoria, Human)
    

    Use

    Tags

    C terminal Venus and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only