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pAc5.1B-lambdaN-HA-DmNot4_248-1050_AG

Plasmid

#148749

Purpose

Insect Expression of DmNot4_248-1050

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

DmNot4_248-1050 (CG31716 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat_57-968-dsRNAres_J

Plasmid

#146690

Purpose

Insect Expression of DmHPat_57-968-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-968-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation A1758G and one non silent G56…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat-del57-499-dsRNAres_J

Plasmid

#146691

Purpose

Insect Expression of DmHPat-del57-499-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat-del57-499-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation A1758G compared to the sequen…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmHPat_57-968-del500-684_J

Plasmid

#146695

Purpose

Insect Expression of DmHPat_57-968-del500-684

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-968-del500-684 (Patr-1 Fly)

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmMe31B-mut2_F

Plasmid

#146272

Purpose

Insect Expression of DmMe31B-mut2

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmMe31B-mut2 (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G and one non silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmHPat_57-968-dsRNAres_H

Plasmid

#146464

Purpose

Insect Expression of DmHPat_57-968-dsRNAres

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmHPat_57-968-dsRNAres (Patr-1 Fly)

Expression

Insect

Mutation

one silent mutation T477C and one non silent muta…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pClathrin-miRFP718nano

Plasmid

#195747

Purpose

Clathrin labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano-clathrin light chain

Tags

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP718nano

Plasmid

#195746

Purpose

Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano-human alpha-tubulin

Tags

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-2X(CMV-eBFP2-3XNLS-pA)

Plasmid

#195867

Purpose

to express nuclear localized eBFP2

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

eBFP2

Use

AAV

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pPG-GPD-TRPC

Plasmid

#190113

Purpose

Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection

Depositor

Perng-Kuang Chang

Article

Chang et al Fungal Genet Biol. 2017 Jul;104:29-37. d

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Linked mRuby3 VLP

Plasmid

#182430

Purpose

Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Inserts

VP2C-GFP-mRuby3
deltaVP1

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1 and GAL10

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Coexpressed mRuby3 VLP

Plasmid

#182431

Purpose

Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Inserts

VP2C-GFP
deltaVP1
VP2C-mRuby3

Use

Synthetic Biology

Expression

Yeast

Promoter

GAL1, GAL10, and GAL7

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-ArgSen (-)P2A (-)NLS

Plasmid

#184690

Purpose

Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen (-)P2A (-)NLS

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-ArgSen (-)NLS

Plasmid

#184698

Purpose

Lentiviral transfer plasmid for expression of ArgSen (-)NLS

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen (-)NLS

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-ArgSen (-)P2A (-)NLS

Plasmid

#184699

Purpose

Lentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLS

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen (-)P2A (-)NLS

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4D41

Plasmid

#185841

Purpose

Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP10CF72 (B11)

Plasmid

#185859

Purpose

Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1- PSkGAL2M3(2*PZ4)>(BamHI)yEGFP>TURA3

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP8EFA

Plasmid

#185854

Purpose

Testing a heat inducible protein degradation tag -DHFR P66L using a green fluorescent protein as the reporter

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PURA3>KlURA3>TAgTEF1-PTEF1>UBI4>RHGSGTMV>DHFRP66L>yEGFP>TPGK1-TURA3

Expression

Yeast

Mutation

DHFR P66L

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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KBB221

Plasmid

#185071

Purpose

SST::ACT2 "SA236"

Depositor

Ken Belanger

Article

Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

Insert

ACT2

Expression

Yeast

Mutation

ACT2-SA236

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KBB215

Plasmid

#185068

Purpose

SST::WAK2 EcoRI/ScaI fragment (secretion confirmed)

Depositor

Ken Belanger

Article

Belanger et al Planta. 2003 Oct;217(6):931-50. doi: 10.

Insert

WAK2

Expression

Yeast

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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