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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,901 results for: cloning

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Showing: 10981 - 11000 of 63901 results

  1. pAV103

    Plasmid
    #63193
    Purpose
    pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Worm and Yeast
    Available since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    63193-plasmid-map-sequence-id-104872

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  2. VanGlow-GL

    Plasmid
    #83342
    Purpose
    for Gateway cloning of promoter elements upstream of a GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Publication
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    GFP::Luciferase(nls)
    Expression
    Insect
    Available since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83342-plasmid-map-sequence-id-158671

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  4. pAV123

    Plasmid
    #63189
    Purpose
    pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63189-plasmid-map-sequence-id-331731

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  5. pSL8215 (dCas12f–σE (sigmaE) system, entry vector)

    Plasmid
    #254272
    Purpose
    Encodes E. coli codon-optimized dCas12f and σE (sigmaE) from Flagellimonas taeanensis driven by constitutive promoters, with BsaI-containing restriction sites for guide RNA cloning
    Depositor
    Samuel H. Sternberg
    Publication
    Hoffmann et al Nature. 2026 Mar 4. doi: 10.1038/s41586-
    Inserts
    dCas12f
    sigma E
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23105
    Available since
    April 6, 2026
    Availability
    Academic Institutions and Nonprofits only
    254272-plasmid-map-sequence-id-505316

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  6. pJRA107

    Plasmid
    #209340
    Purpose
    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.
    Depositor
    Lucia Strader
    Publication
    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi
    Type
    Empty backbone
    Expression
    Plant
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209340-plasmid-map-sequence-id-420461

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  7. pDIRECT_22D

    Plasmid
    #91136
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91136-plasmid-map-sequence-id-182475

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  8. pBEG R2-iBlast-L3 #BGV204

    Plasmid
    #48982
    Purpose
    Supplies the selection marker (blasticidin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
    Depositor
    David Dankort
    Publication
    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:
    Insert
    blasticidin-S deaminase
    Use
    Gateway Cloning
    Tags
    IRES (weak)
    Promoter
    na
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pJH2970

    Plasmid
    #100954
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX marker
    Depositor
    James Haber
    Publication
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100954-plasmid-map-sequence-id-193247

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  10. pANY4-pL18-ccdB

    Plasmid
    #164898
    Purpose
    Two valuable constructs for either IPTG-induced or optimized temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4-pL18-ccdB.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Tags
    His-tag
    Expression
    Bacterial
    Available since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164898-plasmid-map-sequence-id-327989

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Showing: 10981 - 11000 of 63901 results