We narrowed to 18,555 results for: puro
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Plasmid#226165PurposeUsed to integrate 3xFlag-tdMCP-BRaf without MS2-circRNA barcdes into landing pad cell lines containing a single promoter.DepositorInsert3xFlag-tdMCP-BRaf (BRAF Human)
UseBxb1 attb, no promoterTags3xFlag-tdMCP and IRES-mCherry-P2A-PuroRExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mECFP
Plasmid#223954PurposeGateway cloning of genes of interest with an N-terminal V5-mECFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mECFPAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP6
Plasmid#218245Purpose1) Ectopic expression of MAAP6 protein in mammalian cells. 2) Packaging MAAP6 sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein from Adeno-Associated Virus 6 driven by CMV promoter
UseLentiviralPromoterCMVAvailable SinceOct. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pTRE3G-human cGASdeltaN R255E-HA
Plasmid#186894PurposeDoxycycline-dependent expression of human cGAS gene (with R255E mutation) in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with R255E mutation and C-terminal HA tag (Adamts1 Human, Synthetic)
UseRetroviralTagsHAMutationN-terminal truncation, R255EPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-FLAG-human cGASdeltaN-HA
Plasmid#186899PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with N-terminal FLAG and C-terminal HA tag (Adamts1 Human, Synthetic)
UseRetroviralTagsFLAG and HAMutationN-terminal truncationPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-mouse cGAS-GFP
Plasmid#186877PurposeDoxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transductionDepositorAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-FLAG-human cGAS-HA
Plasmid#186849PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS with N-terminal FLAG and C-terminal HA tag (Adamts1 Human)
UseRetroviralTagsFLAG and HAPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP9
Plasmid#218246Purpose1) Ectopic expression of MAAP9 protein in mammalian cells. 2) Packaging MAAP9 sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein from Adeno-Associated Virus 9 driven by CMV promoter
UseLentiviralPromoterCMVAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL187
Plasmid#223680PurposepEF-HA-3xNES-CloningSite-mCherry-puroRDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP-SL08
Plasmid#218247Purpose1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein Variant SL08 driven by CMV promoter
UseLentiviralPromoterCMVAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW57-CMV-MAAP2
Plasmid#218244Purpose1) Ectopic expression of MAAP2 protein in mammalian cells. 2) Packaging MAAP2 sequence into lentivirus for stable expression in mammalian cells.DepositorInsertMembrane-Associated Accessory Protein from Adeno-Associated Virus 2 driven by CMV promoter
UseLentiviralPromoterCMVAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-NRBP1-FL-N-FLAG
Plasmid#222675Purposeoverexpressing full length NRBP1 with N-terminal FLAGDepositorAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TSC22D2-Nterm-C-FLAG
Plasmid#222683Purposeoverexpressing N-terminus truncation of TSC22D2 with C-terminal FLAGDepositorInsertTSC22D2 (TSC22D2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTSC22D2 N-terminus truncationAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-TERF1
Plasmid#222542PurposePiggyBac transposon plasmid for doxycycline inducible expression of TERF1DepositorAvailable SinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PHD2
Plasmid#223552PurposeLentivirus transfer plasmid for expression of full length human PHD2DepositorAvailable SinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PD2529 human beta1 ecto
Plasmid#221398PurposeMammalian expression of human integrin beta1 ectodomainDepositorInsertintegrin beta1 ectodomain (ITGB1 Human)
TagsAVI tag, HRV3C cleavage site, basic coil, HA tag,…ExpressionMammalianMutationcodon optimized for human mature _1 residues Q1 t…Available SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-GFP-SNX9
Plasmid#170551PurposeTo monitor the turnover of SNX9 in lysosomesDepositorInsertSNX9 (SNX9 Human)
UseRetroviralTagsSNX9 in tandem with mCherry and GFPExpressionMammalianAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-CeNL
Plasmid#208839PurposeExpresses FLAG epitope-tagged CeNL "Blue" BRET reporter in mammalian cells.DepositorInsertCeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_CMV:Flag-GeNL
Plasmid#208841PurposeExpresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.DepositorInsertGeNL Nanolantern
UseLentiviralTags1xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only