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pUC19_CoV-2_frD2_ΔE67 3*

Plasmid

#253186

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD2_ΔE67 ORF3*

Expression

Bacterial

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_S Omicron BA.1

Plasmid

#253116

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), Wuhan backbone with Omicron BA.1 spike; chimeric

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Omicron BA.1 spike

Expression

Bacterial

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_ frD2_ΔE67 3* VSVG-P2A-N

Plasmid

#253170

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; VSV glycoprotein fused to N, P2A

Depositor

Thomas Klimkait

Publication

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2 frD2 with VSV-G

Expression

Bacterial

Available since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pQGG005

Plasmid

#231332

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI-ELT4 counterselectable site for BpiI cloning. L2-P15A-Gm-ISceI-ELT4; GG assembly from pOGG006, pOGG009, pNDGG006, pGQ0023, pOGG014. GmR.

Depositor

Barney Geddes

Publication

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-B/c

Plasmid

#227963

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Publication

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

B/c

Expression

Plant

Promoter

35S

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENTR-AtmiR173aTS-B/c

Plasmid

#227964

Purpose

Entry plasmid with AtmiR173aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Publication

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
AtmiR173aTS

Promoter

None

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-NbmiR482aTS-B/c

Plasmid

#227966

Purpose

Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Publication

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
NbmiR482aTS

Promoter

None

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMBS-859

Plasmid

#218009

Purpose

mCerulean fluorescent protein fused to Factor Xa Protease cleavage site and Strep-Tag II

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

mCerulean

Use

Synthetic Biology

Tags

Factor Xa protease cleavage site and Strep-tag II

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-852

Plasmid

#218010

Purpose

Receptor binding domain of SARS-CoV-2

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

RBD (S SARS-CoV-2)

Use

Synthetic Biology

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-864

Plasmid

#218018

Purpose

GST-tag fused to TEV Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

GST-tag

Use

Synthetic Biology

Tags

TEV protease cleavage site

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-845

Plasmid

#218000

Purpose

Strep-Tag II fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Strep-tag II

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-846

Plasmid

#218001

Purpose

3xFLAG-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

3xFLAG-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-847

Plasmid

#218002

Purpose

Myc-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

Myc-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-848

Plasmid

#218003

Purpose

GST-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

GST-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-849

Plasmid

#218004

Purpose

8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

8xHis-tag

Use

Synthetic Biology

Tags

PreScission protease cleavage site

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-850

Plasmid

#218005

Purpose

Receptor binding domain of SARS-CoV-2

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

RBD (S SARS-CoV-2)

Use

Synthetic Biology

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-857

Plasmid

#218007

Purpose

Receptor binding domain of SARS-CoV-2

Depositor

Michael Schroda

Publication

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Insert

RBD (S SARS-CoV-2)

Use

Synthetic Biology

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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KTK_005

Plasmid

#180555

Purpose

Entry vector containing Terminator sequence (Bba_B0015)

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator sequence (Bba_B0015)

Expression

Bacterial

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_047

Plasmid

#180551

Purpose

Entry vector containing sfGFP in E1.3a format

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sfGFP in E1.3a format

Expression

Bacterial

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_110

Plasmid

#180554

Purpose

Entry vector containing 7xhis tag in E1.3b format

Depositor

Tom Ellis

Publication

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

7xhis tag in E1.3b format

Expression

Bacterial

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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