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Neurl1-FLAG/Jag1-HA pIRES

Plasmid

#17337

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pAG221 MDFIC 3'UTR mut

Plasmid

#12051

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N22 3'UTR mut (MDFIC Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
Neurl1G2A-Flag pcDNA3.1

Plasmid

#17320

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Mutation

Gly 2 changed to Ala

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCMV_nLightG2

Plasmid

#221670

Purpose

Expresses nLightG2

Depositor

Tommaso Patriarchi

Article

Rohner et al Nat Methods. 2026 Feb 26. doi: 10.1038/s

Insert

nLightG2

Expression

Mammalian

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV_nLightR2

Plasmid

#221671

Purpose

Expresses nLightR2

Depositor

Tommaso Patriarchi

Article

Rohner et al Nat Methods. 2026 Feb 26. doi: 10.1038/s

Insert

nLightR2

Expression

Mammalian

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Split SNORA70

Plasmid

#200644

Purpose

Expressed a eGFP sequence where a chimeric intron with the sequence reference of SNORA70 (NR_000011) was inserted

Depositor

Claire Francastel

Article

Bogard et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

Split GFP with chimeric intron containing SNORA70

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Split SNORA40

Plasmid

#200645

Purpose

Expressed a eGFP sequence where a chimeric intron with the sequence reference of SNORA40 (NR_002973.1) was inserted

Depositor

Claire Francastel

Article

Bogard et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

Split GFP with chimeric intron containing SNORA40

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2

Plasmid

#221082

Purpose

Plasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-msfGFP[r5M]_ACC-mEBFP2

Use

Synthetic Biology

Promoter

CMV, T7

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 EGFP sgRNA4

Plasmid

#164934

Purpose

Expresses EGFP sgRNA4 in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 NT sgRNA5

Plasmid

#164930

Purpose

Expresses Non-targeting sgRNA5 control in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

Non-targeting sgRNA5 (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGex-6p2_ATRX_K1033R

Plasmid

#221378

Purpose

Expresses ATRX_K1033R in bacterial cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Transcriptional regulator ATRX (ATRX Human)

Expression

Bacterial

Mutation

Changed lysine 1033 to arginine

Promoter

tac

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGex-6p2_FANCM_K819R

Plasmid

#221380

Purpose

Expresses FANCM_K819R in bacterial cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Fanconi anemia group M protein (FANCM Human)

Expression

Bacterial

Mutation

Changed lysine 819 to arginine

Promoter

tac

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEYFP_C1_ATRX

Plasmid

#221383

Purpose

Expresses ATRX in mammalian cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Transcriptional regulator ATRX (ATRX Human)

Expression

Mammalian

Mutation

K536N (Please see depositor comments)

Promoter

CMV

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCPH2A.Z-GS3

Plasmid

#204758

Purpose

Expression of Cas9 and human H2A.Z

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

Insert

H2AZ1 (H2AZ1 Human)

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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TDP-43 mRuby K84ONBK

Plasmid

#216226

Purpose

TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

Depositor

Jennifer Lee

Article

Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

Insert

Transactive response DNA binding protein of 43 kDa (TARDBP Human)

Expression

Mammalian

Mutation

Lysine 84 mutated to a TAG stop codon

Promoter

CMV Promoter

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-NFAT5-WT

Plasmid

#215597

Purpose

For overexpression of Gal4-DBD NFAT5 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NFAT5 (NFAT5 Human)

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_05-mEGFP_WRPE-SV40

Plasmid

#194689

Purpose

hSyn1 driven expression of the calcium recorder Caprola_05 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_05-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_04

Plasmid

#194665

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_04 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_07

Plasmid

#194668

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_07 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_07

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_08

Plasmid

#194669

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_08 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_08

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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