We narrowed to 16,086 results for: nes

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piwi_sgRNA

Plasmid

#186653

Purpose

piwi sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Piwi sgRNA Plasmid (piwi Fly)

Expression

Insect

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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psgRNA-2xMS2-lacO

Plasmid

#181909

Purpose

Single guide RNA with 2XMS2 loops targeting bacterial lac operator

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

sgRNA-2XMS2-lacO

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC_dCas9_sgRNA Td2

Plasmid

#176258

Purpose

pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

dead spCas9

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Mutation

H840A and D10A mutations on spCas9 to inactivate …

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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B-HypaSpCas9

Plasmid

#126763

Purpose

Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

B-HypaSpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

N692A; M694A; Q695A; H698A; amino acids 1005-1013…

Promoter

CBh

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS-Hypa-nCas9n-A69

Plasmid

#125645

Purpose

micro-injection of poly-adenylated Hypa-nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

Hypa-nCas9n

Use

Rna in vitro transcription vector

Mutation

N692A, M694A, Q695A, H698A from wild-type Cas9

Promoter

T3

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCSD_SpCas9MT2-NLS-3xHA-NLS-SaCas9WT-2xNLS

Plasmid

#107321

Purpose

Expresses SpCas9 (R1333S) fused to SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 (R1333S) is fused to SaCas9

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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6mSPL11F15R14::pBIB-BAS (6mSPL11)

Plasmid

#27387

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL11 mutant (AT1G27360 Mustard Weed)

Use

Used to create transgenic lines

Mutation

miR156 target sites of SPL11 mutated

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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6mpSPL10F13R13::pBIB-BAS (6mSPL10)

Plasmid

#27408

Depositor

David Bartel

Article

Nodine et al Genes Dev. 2010 Dec 1. 24(23):2678-92.

Insert

SPL10 mutant (AT1G27370 Mustard Weed)

Use

Used to create transgenic lines

Mutation

miR156 target sites of SPL10 mutated

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only

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Y344C

Plasmid

#24433

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, Y344C

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
K222C

Plasmid

#24438

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
L4C

Plasmid

#24437

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, L4C

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only
S330C

Plasmid

#24439

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S/C421A, "S…

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only
K339CLM S330C

Plasmid

#24441

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S, S330C

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only
K343CLM A337C

Plasmid

#24442

Depositor

Ron Vale

Article

Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

Insert

Kinesin (KIF5B Human)

Tags

6XHis and GFP

Expression

Bacterial

Mutation

C7S/C65A/C168A/C174S/C294A/C330S, A337C

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F49

Plasmid

#23073

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysines 8 and 16 to Arginine, …

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F51

Plasmid

#23075

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H4 changed Lysines 5 and 12 to Glutamine,…

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pLenti6.3-D133p53a (R273H)

Plasmid

#259518

Purpose

Expresses a human p53 isoform (containing a cancer-associated mutation) in mammalian cells

Depositor

Curtis Harris, Izumi Horikawa

Article

Joruiz et al Cell Death Dis. 2024 Jun 27;15(6):454. d

Insert

Delta133p53alpha

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed Arginine 273 (numbering on NM_000546.6) t…

Available Since

Aug. 5, 2026

Availability

Academic Institutions and Nonprofits only

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p_sc-eVIP-delta-Puro-EGFP-PAX3

Plasmid

#249472

Purpose

Transfer plasmid for stable transgene expression with 2nd or 3rd generation lentiviral systems

Depositor

Nils Blüthgen

Article

Hollek et al Mol Syst Biol. 2026 Jan 19. doi: 10.1038

Insert

PAX3 (PAX3 Human)

Use

Lentiviral

Promoter

EF-1a

Available Since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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p_sc-eVIP-delta-Puro-EGFP-MAP2K1

Plasmid

#249464

Purpose

Transfer plasmid for stable transgene expression with 2nd or 3rd generation lentiviral systems

Depositor

Nils Blüthgen

Article

Hollek et al Mol Syst Biol. 2026 Jan 19. doi: 10.1038

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Promoter

EF-1a

Available Since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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p_sc-eVIP-delta-Puro-EGFP-CTNNB1

Plasmid

#249451

Purpose

Transfer plasmid for stable transgene expression with 2nd or 3rd generation lentiviral systems

Depositor

Nils Blüthgen

Article

Hollek et al Mol Syst Biol. 2026 Jan 19. doi: 10.1038

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral

Promoter

EF-1a

Available Since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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