We narrowed to 16,278 results for: nes

Showing: 11041 - 11060 of 16278 results

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pYihI-N-term K-R

Plasmid

#233091

Purpose

Expression of GST-YihI with N-term lysines (K) mutated to arginine (R)

Depositor

Michael Downey

Publication

Baijal et al mBio. 2025 Aug 13;16(8):e0050025. doi: 1

Insert

YihI with N-term lysines (K) mutated to arginine (R)

Tags

GST

Expression

Bacterial

Mutation

N-terminal lysine residues mutated to arginine

Available since

May 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-CsHPAD-AE

Plasmid

#226982

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coli

Depositor

Emily Balskus

Publication

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD-AE

Tags

His6 (on backbone)

Expression

Bacterial

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pmcb306-GFP-Rab12-mito

Plasmid

#207349

Purpose

Mammalian expression of Rab12-mito

Depositor

Suzanne Pfeffer

Publication

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

Rab12-mito (RAB12 Human)

Expression

Mammalian

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ10 PPM1H

Plasmid

#207335

Purpose

Mammalian expression of HA-Δ10 PPM1H

Depositor

Suzanne Pfeffer

Publication

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ10

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ20 PPM1H

Plasmid

#207336

Purpose

Mammalian expression of HA-Δ20 PPM1H

Depositor

Suzanne Pfeffer

Publication

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ20

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ34 PPM1H

Plasmid

#207337

Purpose

Mammalian expression of HA-Δ34 PPM1H

Depositor

Suzanne Pfeffer

Publication

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ34

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ37 PPM1H

Plasmid

#207338

Purpose

Mammalian expression of HA-Δ37 PPM1H

Depositor

Suzanne Pfeffer

Publication

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ37

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ44 PPM1H

Plasmid

#207339

Purpose

Mammalian expression of HA-Δ44 PPM1H

Depositor

Suzanne Pfeffer

Publication

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ44

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV5D HA-Δ71 PPM1H

Plasmid

#207340

Purpose

Mammalian expression of HA-Δ71 PPM1H

Depositor

Suzanne Pfeffer

Publication

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

PPM1H (PPM1H Human)

Tags

HA

Expression

Mammalian

Mutation

Δ71

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HypaSpCas9-NLS-6xHis

Plasmid

#207384

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N692A, M694A, Q695A, H698A

Promoter

T7

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-evoSpCas9-NLS-6xHis

Plasmid

#207389

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

evoSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

M495V, Y515N, K526E, R661Q

Promoter

T7

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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Yeast_Target-AID2S

Plasmid

#188647

Purpose

Expressing Target-AID2S in yeasts

Depositor

Keiji Nishida

Publication

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Tags

UGI

Expression

Yeast

Mutation

D10A for SpCas9

Promoter

pGal1

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCbh-SpyCas9-2A-puro-pA_U6-BbsI

Plasmid

#149347

Purpose

Mammalian expression vector for SpyCas9 and puromycin resistance

Depositor

Ralf Kuehn

Publication

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

SpyCas9

Use

CRISPR

Tags

Flag tag

Expression

Mammalian

Promoter

Cbh

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pPJ169(JPUB_006769)

Plasmid

#83835

Purpose

Carries NEW operon 5 final

Depositor

Harry Beller

Publication

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Inserts

Kuste3340
Kuste3350

Promoter

op5_3350_for and op5_BsaI_3340_for

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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ARL13B-(ΔPRR)-GFP

Plasmid

#246846

Purpose

Express Arl13B (resistance to sgRNA) with deletion of Proline-Rich Region (PRR) domain, tagged with GFP

Depositor

Lei Lu

Publication

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deleted PRR domain

Available since

April 14, 2026

Availability

Academic Institutions and Nonprofits only

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ARL13B-(Y86C)-GFP

Plasmid

#246840

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with Y86C mutation

Depositor

Lei Lu

Publication

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Tyrosine 86 to Cysteine

Available since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(R79Q)-GFP

Plasmid

#246839

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with R79Q mutation

Depositor

Lei Lu

Publication

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Arginine 79 to Glutamine

Available since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(C8C9- SS)-GFP

Plasmid

#246842

Purpose

Express Arl13B (resistance to sgRNA), tagged with GFP, with C8C9-SS mutation

Depositor

Lei Lu

Publication

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

changed Cysteine 8 and 9 to Serine

Available since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ARL13B-(ΔIDR)-GFP

Plasmid

#246845

Purpose

Express Arl13B (resistance to sgRNA) with deletion of Intrinsically Disordered Region, tagged with GFP

Depositor

Lei Lu

Publication

Mahajan et al Sci Rep. 2025 Jul 24;15(1):26980. doi: 1

Insert

Arl13B (ARL13B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

deleted IDR domain

Available since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW121_WT-Cas9_U6-entry

Plasmid

#244818

Purpose

Mammalian expression of WT Cas9 with Golden Gate-compatible sgRNA spacer cassette

Depositor

Jennifer Doudna

Publication

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Promoter

CAG, U6

Available since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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