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VN-dCasE-VC-GK

Plasmid

#164277

Purpose

Express VN-dCasE-VC protein in mammalian cells. This plasmid is also referred to as as VN-dEcCas6-VC-GK in the publication.

Depositor

Chunyu Han

Article

Gao et al Nucleic Acids Res. 2022 May 6;50(8):e46.

Insert

VN-dCasE-VC

Expression

Mammalian

Mutation

H20A in EcCasE

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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Ntom-Venus-ssrA

Plasmid

#223688

Purpose

PhoBIT1 component; Venus-tagged ssrA tag fused with Ntom for mitochondrial tethering

Depositor

Yubin Zhou

Article

Lee et al Nat Commun. 2025 Jul 28;16(1):6940. doi:

Insert

ssrA peptide

Tags

mVenus

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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mCh-sspB(LOV2)

Plasmid

#223689

Purpose

PhoBIT1 component; mCherry-tagged sspB with LOV2 insertion

Depositor

Yubin Zhou

Article

Lee et al Nat Commun. 2025 Jul 28;16(1):6940. doi:

Insert

sspB(N)-LOV2-sspB(C)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJM220-PhoA-mNeongreen-J23101-mScarletI

Plasmid

#240201

Purpose

MiniTn7 donor plasmid for constructing a ratiometric reporter for P limitation in Pseudomonas synxantha. A strong Anderson promoter (J23101) drives expression of mScarlet-I

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2024 Jan 19;13(1):384-39

Inserts

mNeongreen
mScarlet-I

Promoter

J23101 and PphoA

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pJM220-PphoA-mNeongreen-J23116-mScarletI

Plasmid

#240200

Purpose

MiniTn7 donor plasmid for constructing a ratiometric reporter for P limitation in Pseudomonas synxantha. A weak Anderson promoter (J23116) drives expression of mScarlet-I

Depositor

Richard Murray

Article

Larsson et al ACS Synth Biol. 2024 Jan 19;13(1):384-39

Inserts

mNeongreen
mScarlet-I

Promoter

J23116 and PphoA

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2661

Plasmid

#234500

Purpose

Linker-Cno_mNeonGreen-CBP-2xFLAG-CnU6-sgRNA-NEOR

Depositor

Hiten Madhani

Article

Nalley et al G3 (Bethesda). 2025 Aug 6;15(8):jkaf118.

Insert

mNeonGreen-CBP-2XFLAG-NEO-PCnU6-sgRNA_scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBTK1129

Plasmid

#236752

Purpose

BTK Type 2-3 fluorescent protein cassette for golden gate assembly. Improved version of pBTK1055

Depositor

Jeffrey Barrick

Article

Lariviere et al mBio. 2024 Sep 11;15(9):e0139224. doi: 1

Insert

Type 2-3 GFP cassette

Use

Synthetic Biology

Promoter

CP25

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pIAR007

Plasmid

#217022

Purpose

deletion of aldB-II

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_2679 (pedH) and PP_2681 (pqqD-II)

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pIAR008

Plasmid

#217023

Purpose

deletion of amaB

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_5257 (amaA) and PP_5259

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pIAR009

Plasmid

#217024

Purpose

deletion of aldA

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_2486 (xenC) and PP_2488 (sad_I)

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pIAR010

Plasmid

#217025

Purpose

deletion of modABC

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_3827 (yrpB) and PP_3831

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pIAR014

Plasmid

#217026

Purpose

deletion of ior_operon

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_3620 (ycaO) and PP_3624

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pIAR015

Plasmid

#217027

Purpose

deletion of vanillic_acid_shunt

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_3353 and PP_3356 (fcs)

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pIAR001

Plasmid

#217016

Purpose

deletion of vdh

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequence of PP_3356 (fcs) and PP_3358 (ech)

Use

Bacterial gene replacement

Tags

none

Mutation

none

Promoter

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pIAR002

Plasmid

#217017

Purpose

deletion of aldB-I

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_0544 and PP_0546

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pIAR003

Plasmid

#217018

Purpose

deletion of PP_1948

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_1947 and PP_1949

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pIAR004

Plasmid

#217019

Purpose

deletion of areA(calA)

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_2425 and PP_5521

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pIAR005

Plasmid

#217020

Purpose

deletion of sadII

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_3150 and PP_3152 (yneJ)

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pIAR006

Plasmid

#217021

Purpose

deletion of calB

Depositor

Gregg Beckham

Article

Ruhl et al Microb Biotechnol. 2025 May;18(5):e70152

Insert

partial sequences of PP_5119, PP_5121, and PP_5122

Use

Bacterial gene replacement

Tags

none

Mutation

none

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pXYB106

Plasmid

#239640

Purpose

Expression vector of Ribozyme split GFPuv with tEvopreq motif

Depositor

Xiaohan Yang

Article

Liu et al Plant Biotechnol J. 2025 May;23(5):1640-

Insert

GFPuvn_Y72-Ribozyme-2nd_GFPuvc+tEvopreq

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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