We narrowed to 15,981 results for: LEA

1. human KANK1 Ankyrin Repeats pET151

   Plasmid

   #164053

   Purpose

   Expresses human KANK1 Ank domain in bacterial cells

   Depositor

   Ben Goult

   Insert

   Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
   

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMEXC3GH (Apr)

   Plasmid

   #110083

   Purpose

   Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS-TarHom-Tol2-FRT-GalK-cryaa-CFP

   Plasmid

   #73200

   Purpose

   BAC targeting cassette for pTARBAC2 with GalK selection marker and lens-specific transgenesis marker Cerulean

   Depositor

   Holger Knaut

   Article

   Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

   Insert

   CFP
   

   Available Since

   March 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSDMA66

   Plasmid

   #128355

   Purpose

   gRNA ribozyme construct with Cryptococcus neoformans ACT1 promter and TRP1 terminator. Following ribozyme cleavage, a gRNA targeting ADE2 is liberated.

   Depositor

   James Fraser

   Article

   Arras et al PLoS One. 2016 Oct 6;11(10):e0164322. do

   Insert

   Noureothricin (NAT)
   

   Use

   Unspecified

   Promoter

   ACT1

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBW829_pCAG-pExpr-LUT

   Plasmid

   #87554

   Purpose

   Encodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinases

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   EGFP
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICSL50016

   Plasmid

   #50315

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
   

   Use

   Plant expression

   Mutation

   Codon-optimised for A. thaliana. BsaI/BbsI sites …

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4258326

   Plasmid

   #154793

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 surface glycoprotein (Spike protein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;V5

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pNLS-LSSmKate1-N1

   Plasmid

   #31870

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

   Insert

   NLS
   

   Expression

   Mammalian

   Mutation

   K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

   Promoter

   CMV

   Available Since

   Sept. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046574

   Plasmid

   #148976

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable thrombin;3xFLAG

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pGAL1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. 5THA (Gemin5)

    Plasmid

    #101890

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Gemin5 (GEMIN5 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis, Thrombin cleavage

    Promoter

    polyhedrin

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. WDR5

    Plasmid

    #36900

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5 (WDR5 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    mutation version of plasmid #60121 3UR4

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pJA59

    Plasmid

    #59932

    Purpose

    gRNA for cleavage at unc-109(n499) locus in C elegans

    Depositor

    Andrew Fire

    Article

    Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

    Insert

    unc-109(n499) gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Sept. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGBW-m4046332

    Plasmid

    #145413

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. LMNB2

    Plasmid

    #36896

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    LMNB2 (LMNB2 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains Laminb2 Ig-like domain, amino acid resid…

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pJOG140

    Plasmid

    #105399

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    PKI nuclear export signal
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJOG141

    Plasmid

    #105400

    Purpose

    Synthetic biology

    Depositor

    Johannes Stuttmann

    Article

    Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

    Insert

    SV40 nuclear localization signal
    

    Use

    Synthetic Biology

    Mutation

    BsaI/ BpiI restriction sites removed

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. csr-1_son-1_mScarlet

    Plasmid

    #191745

    Purpose

    Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    NCU04288 (NCU04288 Neurospora crassa)
    

    Tags

    NCU04502 promoter and mScarlet

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4046437

    Plasmid

    #145415

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGBW-m4046431

    Plasmid

    #145526

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pet11a-ABLE

    Plasmid

    #158627

    Purpose

    Amp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expression

    Depositor

    William DeGrado

    Article

    Polizzi et al Science. 2020 Sep 4;369(6508):1227-1233.

    Insert

    Apixaban-Binding Helical Bundle
    

    Tags

    6x His-tag and TEV cleavage tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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