We narrowed to 14,841 results for: flag

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pEBTet-SNAP-α-tubulin

Plasmid

#136791

Purpose

Mammalian expression of the centrosomal protein _-tubulin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-TUBA (DNMBP Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Centrin2

Plasmid

#136784

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin2 (CETN2 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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BII-ChPtinGW

Plasmid

#133377

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-dnTCF4-ires-GFP

Plasmid

#133378

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

dnTCF4 (TCF4 Human)

Tags

2x Flag

Expression

Mammalian

Mutation

deltaN31

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-(BsaI)_CBh-UN-Cas9-T2A-mCherry

Plasmid

#135013

Purpose

Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9, linked to mCherry via a T2A peptide

Depositor

Yosef Shaul

Article

Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

Insert

humanized S. pyogenes Cas9

Tags

3x Flag, 3xFLAG (N terminal on insert), NLS, and …

Expression

Mammalian

Mutation

126aa domain from HSV-1 UL12 fused to the N-termi…

Promoter

CBh

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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BII-CBtW

Plasmid

#133372

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAWp63-clone32 (Opti-SpCas9)

Plasmid

#131736

Purpose

Expresses Opti-SpCas9 in mammalian cells

Depositor

Alan Siu Lun Wong

Article

Choi et al Nat Methods. 2019 Aug;16(8):722-730. doi

Insert

Opti-SpCas9

Use

Lentiviral

Tags

Flag

Mutation

R661A+K1003H

Promoter

EFS

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)- e18 LOF

Plasmid

#131729

Purpose

Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

Depositor

Alberto Ciccia

Article

Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

Insert

e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-st1CAS9-pA

Plasmid

#131542

Purpose

Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

Insert

Codon optimized st1Cas9

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-T3-SpCAS9-NG-pA

Plasmid

#131465

Purpose

Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

Depositor

Wataru Fujii

Article

Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

Insert

Codon optimized SpCas9-NG

Use

CRISPR

Tags

Flag, NLS, and polyA

Expression

Mammalian

Available Since

Nov. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a_ TET1 CXXC only

Plasmid

#124084

Purpose

Expression of CXXC domain only form of TET1

Depositor

Silvia Monticelli

Article

Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

Insert

TET1 - CXXC domain (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF1 alpha

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAH235

Plasmid

#121145

Purpose

Cas9 expression controlled by the nmt41 promoter

Depositor

Katsunori Tanaka

Article

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Insert

humanized Streptococcus pyogenes Cas9

Use

CRISPR

Tags

Flag

Expression

Yeast

Promoter

nmt41

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-MUS2-1246-vpr

Plasmid

#115841

Purpose

Encodes codon optimized 3xFLAG-SIVMUS21246 Vpr (EF070329) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMUS21246 Vpr (EF070329)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-GRV vpr

Plasmid

#115827

Purpose

Encodes codon optimized 3xFLAG-SIVGRV Vpr (M66437) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGRV Vpr (M66437)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-GSN-CN71 vpr

Plasmid

#115830

Purpose

Encodes codon optimized 3xFLAG SIVGSN-CN71 Vpr (AF468658) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVGSN-CN71 Vpr (AF468658)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-DEN vpr

Plasmid

#115829

Purpose

Encodes codon optimized 3xFLAG SIVDEN Vpr (AJ580407) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVDEN Vpr (AJ580407)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-AGM-MAL vpr

Plasmid

#115828

Purpose

Encodes codon optimized 3xFLAG-SIVAGM Vpr (MAL_ZMB) (LC114462) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVAGM Vpr (MAL_ZMB) (LC114462)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-ASC vpr

Plasmid

#115824

Purpose

Encodes codon optimized 3xFLAG-SIVASC Vpr (KJ461715) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVASC Vpr (KJ461715)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-DEB vpr

Plasmid

#115823

Purpose

Encodes codon optimized 3xFLAG-SIVDEB Vpr (FJ919724) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVDEB Vpr (FJ919724)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPS-SIV-LST vpr

Plasmid

#115818

Purpose

Encodes codon optimized 3xFLAG-SIVLST Vpr (AF188116) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVLST Vpr (AF188116)

Use

Lentiviral

Tags

3x-FLAG

Promoter

SFFV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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