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pHACK-Sparse.FRT10-GAL4.DBD

Plasmid

#232833

Purpose

Sparse.FRT10 GAL4.DBD for HACK system

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4.DBD (GAL4 Budding Yeast)

Use

CRISPR

Expression

Insect

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBP-Sparse.FRT10-GAL4.DBD

Plasmid

#232831

Purpose

Enhancer-free Sparse.FRT10 GAL4.DBD with ccdB for Gateway

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4.DBD (GAL4 Budding Yeast)

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-1_M13ps

Plasmid

#231409

Purpose

M13 phagemid that expresses sgRNA-1 from a strong constitutive promoter.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-1

Use

Synthetic Biology

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLY071c

Plasmid

#227940

Purpose

SICLOPPS precursor for cyclo-CHVVGVI

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CHVVGVI

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLY071e

Plasmid

#227941

Purpose

SICLOPPS precursor for cyclo-CDLGVFR

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CDLGVFR

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLY090a

Plasmid

#227938

Purpose

SICLOPPS precursor for cyclo-CRVWCAR

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CRVWCAR

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLY090b

Plasmid

#227939

Purpose

SICLOPPS precursor for cyclo-CRVYQVL

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Evolved Ssp SICLOPPS-CRVYQVL

Expression

Bacterial

Mutation

Evolved Ssp split inteins with multiple mutations…

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT017

Plasmid

#225168

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (AmpR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pIND4-RC-C2-M210TAG+M252V-oCNF

Plasmid

#222995

Purpose

Expresses 3 subunits of photosynthetic reaction center from R. sphaeroides and aaRS-tRNA pair for ortho-phenylalanine variants

Depositor

Steven Boxer

Inserts

puhA
pufL
pufM
Phe variants PylRs
Mm Pyl tRNA for oF incorporation

Tags

6xHis

Mutation

changed Tyr M210 to amber stop codon (TAG) for am…

Promoter

rrnB

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT024

Plasmid

#225175

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (SmR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT023

Plasmid

#225174

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (SmR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT019

Plasmid

#225170

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (TetR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT020

Plasmid

#225171

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (TetR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT022

Plasmid

#225173

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (GmR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT016

Plasmid

#225167

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (ChlR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT015

Plasmid

#225166

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (ChlR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pHBJT018

Plasmid

#225169

Purpose

Low copy cloning vector with same multiple cloning site as pUC18 and pSU18 (AmpR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0- Gfap-TagIN-mCherry

Plasmid

#196490

Purpose

To tag endogenous mouse GFAP with mCherry

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Gfap-GeneTRAP

Plasmid

#196492

Purpose

To KO and genetrap mouse GFAP simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry-CAAX
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Vamp2-GeneTRAP

Plasmid

#196493

Purpose

To KO and genetrap mouse VAMP2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry-CAAX
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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