We narrowed to 2,908 results for: quo
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Plasmid#136298Purposeexpresses ABE8.17-m in mammalian cellsDepositorInsertABE8.17-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
1179_pAAV-U6-BbsI-gRNA-CB-EmGFP
Plasmid#89060PurposeAAV-gRNA cloning vector with GFP reporterDepositorInsertEmGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterChicken beta actin with partial CMV enhancerAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
Gβ-2A-cpV-Gγ2-IRES-Gαi2-mTq2
Plasmid#69624PurposeA FRET sensor for Galphai2 activation, encoded on a single plasmid.DepositorInsertsGbeta-2A-cpV-Ggamma2
Galphai2-mTurquoise2
UseTagsciruclar permutated Venus (cp173V) and mTurquoise…ExpressionMammalianMutationPromoterCMV and CMV (IRES)Available sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABE8.13-m
Plasmid#136296Purposeexpresses ABE8.13-m in mammalian cellsDepositorInsertABE8.13-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pCamk2a-AcGFP
Plasmid#133732PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianMutationPromoterpCamk2a and pTRE3G-BiAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pCMV-AcGFP
Plasmid#131008PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianMutationPromoterpCMV and pTRE3G-BiAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2
Plasmid#69623PurposeA FRET sensor for Galphai1 activation, encoded on a single plasmid.DepositorInsertsGbeta-2A-cpV-Ggamma2
Galphai-mTurquoise2
UseTagsciruclar permutated Venus (cp173V) and mTurquoise…ExpressionMammalianMutationPromoterCMV and CMV (IRES)Available sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-GFP
Plasmid#40592DepositorInsertGFP-S65T
UseMouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionBacterial and MammalianMutationConstruct contains a mutated, humanized hGFP(S65T…PromoterhGFAP promoterAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pGFAP-AcGFP
Plasmid#133733PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a GFAP promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianMutationPromoterpGFAP and pTRE3G-BiAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
ABE8.20-d
Plasmid#136301Purposeexpresses ABE8.20-d in mammalian cellsDepositorInsertABE8.20-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterCMVAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMVS102_P3-GFP-NATMX6
Plasmid#99048PurposeEGFP reporter with six binding sites for the Zif268 DNA binding domainDepositorInsertsMcIsaac 2014 P3 promoter
eGFP
clonNAT resistance
UseTagsExpressionYeastMutationGAL1 promoter with 4 GAL4 sites removed and repla…PromoterMcIsaac 2014 P3 promoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gβ-2A-cpV-Gγ2-IRES-Gαi3-mTq2
Plasmid#69625PurposeA FRET sensor for Galphai3 activation, encoded on a single plasmid.DepositorInsertsGbeta-2A-cpV-Ggamma2
Galphai3-mTurquoise2
UseTagsciruclar permutated Venus (cp173V) and mTurquoise…ExpressionMammalianMutationPromoterCMV and CMV (IRES)Available sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-flex-Clover
Plasmid#135177PurposeAAV expression of TRE(TRE3G)-driven, Cre-dependent, Clover expression for fluorescent lablingDepositorInsertsClover
TRE3G
UseAAVTagsExpressionMammalianMutationa variant of GFPPromoterTRE3GAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
H2B-mAvicFP1
Plasmid#129511PurposeExpresses H2B-mAvicFP1 in mammalian cells (human histone 2B)DepositorInsertH2B-mAvicFP1
UseTagsExpressionMammalianMutationmAvicFP1 fused to the C terminus of human histone…PromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ABE8.17-d
Plasmid#136299Purposeexpresses ABE8.17-d in mammalian cellsDepositorInsertABE8.20-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR P2R-P3-EGFP
Plasmid#48349PurposeContributes the coding sequence for EGFP as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.DepositorInsertEGFP
UseGateway entry vectorTagsExpressionMutationAminoacid 40 is valine in our sequence, while it …PromoternoneAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
ABE8.8-d
Plasmid#136295Purposeexpresses ABE8.8-d in mammalian cellsDepositorInsertABE8.8-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ oDam_f_GFPoNLS
Plasmid#85819PurposeiDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertoDam_f_GFPoNLS
UseTagsoNLS (optimized nuclear localization signal) C te…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP
Plasmid#89061PurposeAAV-gRNA targeting the murine Ldlr geneDepositorInsertEmGFP
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterChicken beta actin with partial CMV enhancerAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only