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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,326 results for: bon;

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Showing: 5591 - 5600 of 18326 results

  1. pCJ200

    Plasmid
    #162673
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C and a sequence Y75-F104 to introduce a 6th…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162673-plasmid-map-sequence-id-321521

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  2. pCJ210

    Plasmid
    #162683
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C, G489C, S530C, and a sequence Y75-F104 to i…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162683-plasmid-map-sequence-id-321486

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  3. Fibronectin-human-mutated N-terminal (N) O-Glycosylation site

    Plasmid
    #120404
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which the first O-glycosylation site was mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Expression
    Mammalian
    Mutation
    Containing complete variable region with a mutati…
    Promoter
    CMV
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120404-plasmid-map-sequence-id-232440

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  4. Fibronectin-human-mutated V2 O-Glycosylation site

    Plasmid
    #120405
    Purpose
    enables eukaryotic expression of human plasma fibronectin in which the second O-glycosylation site was mutated from threonine to serine
    Depositor
    Inaam Nakchbandi
    Article
    Sens et al J Bone Miner Res. 2017 Jan;32(1):70-81.
    Insert
    Fibronectin (FN1 Human)
    Expression
    Mammalian
    Mutation
    Contains complete variable region with a mutation…
    Promoter
    CMV
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120405-plasmid-map-sequence-id-232458

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Showing: 5591 - 5600 of 18326 results