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Showing: 11201 - 11220 of 19683 results

  6. pCR4_miniFXN_8

    Plasmid
    #234361
    Purpose
    Mini FXN plasmid with a partial deletion of the 5' UTR and a large deletion of intron 1 in FXN. Does not contain GAA repeats.
    Depositor
    Marek Napierala
    Publication
    Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85
    Insert
    FXN (FXN Human)
    Use
    Cloning
    Tags
    Flag Tag
    Mutation
    There is a partial deletion in the 5'UTR of …
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    234361-plasmid-map-sequence-id-464762

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  7. pCY5k

    Plasmid
    #160294
    Purpose
    Yeast CRISPR plasmid targeting the kanMX cassette
    Depositor
    Amir Aharoni
    Publication
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160294-plasmid-map-sequence-id-464601

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  14. TMEM16F-I521K-peGFP-N1

    Plasmid
    #228588
    Purpose
    Expresses TMEM16F-I521K-peGFP in mammalian cells
    Depositor
    Huanghe Yang
    Publication
    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    I521K plus a 3 amino acid N-terminal truncation (…
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228588-plasmid-map-sequence-id-454815

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  16. psiCHECK-VIM270-gCM

    Plasmid
    #215868
    Purpose
    This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the knockdown efficiency of siRNA.
    Depositor
    Kumiko Ui-Tei
    Publication
    Iribe et al ACS Omega. 2017 May 31;2(5):2055-2064. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    target site which is completely complementary to guide strand of siRNA for vimentin is inserted to 3'UTR of RLuc (VIM Human)
    Expression
    Mammalian
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215868-plasmid-map-sequence-id-431664

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  18. pETDuet-1-6xHis-TEV-Nsp8

    Plasmid
    #201022
    Purpose
    Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.
    Depositor
    Sharon Rozovsky
    Publication
    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d
    Insert
    non-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    May 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    201022-plasmid-map-sequence-id-394460

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  20. Lenti_EGFP-P2A-siRNAresHAUS6_IRES_Blast

    Plasmid
    #182887
    Purpose
    Transfer vector for production of lentivirus. Expresses EGFP-P2A-HAUS6 (siRNA resistant version)
    Depositor
    Daniel Gerlich
    Publication
    David et al J Cell Biol. 2019 Jul 1;218(7):2150-2168
    Insert
    HAUS 6 (HAUS6 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    silet mutations in aa 197 - 203 to confer resista…
    Promoter
    EF1alpha core
    Available since
    July 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    182887-plasmid-map-sequence-id-367932

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Showing: 11201 - 11220 of 19683 results