We narrowed to 57,977 results for: cmv

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hRad51(K133R)–Cas9(D10A)

Plasmid

#125568

Purpose

Expresses the Rad51(K133R)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(K133R)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-HNF4α

Plasmid

#139305

Purpose

Knocking down of human HNF4α through shRNA and amiRNA targeting HNF4α

Depositor

Claes Wadelius

Article

Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

Insert

shRNA and amiRNA against HNF4α

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-CRY2-mCh-superPLDx10

Plasmid

#188986

Purpose

A lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CRY2 and mCherry

Expression

Mammalian

Mutation

F163L, K249R, G328S, G381V, G429D, D480G

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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SETMAR-Cas9

Plasmid

#186701

Purpose

Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

SETMAR-Cas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGinB-8X(GGS)-dCas9-FLAG-NLS

Plasmid

#81205

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pBK-YE2-BE3

Plasmid

#85176

Purpose

Expresses YE2-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YEE-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-BFP

Plasmid

#106282

Purpose

A version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.

Depositor

Jay Shendure

Article

Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

Insert

CMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBK-EE-BE3

Plasmid

#85175

Purpose

Expresses EE-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

EE-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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RFP-FKBP-PRPF38A

Plasmid

#187968

Purpose

expresses PRPF38A with N-terminal tags RFP and FKBP

Depositor

Markus Wahl

Article

Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

Insert

Prp38A (PRPF38A Human, Synthetic)

Use

Cmv promoter

Tags

mRFP-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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hRad51(A190L-A192L)–Cas9(D10A)

Plasmid

#125562

Purpose

Expresses the Rad51(A190L-A192L)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(A190L A192L)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGinB-8X(GGS)-dCas9-FLAG-NLS_SpecR

Plasmid

#81206

Purpose

CMV promoter driving expression of GinB catalytic domain fused with linker to dCas9 in the order shown. Has a FLAG and NLS tag on the c-terminus. Spec resistance

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

pGinB-8X(GGS)-dCas9-FLAG-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pRSM32.2

Plasmid

#101635

Purpose

Encodes the fluorescent protein Rosmarinus under a CMV promoter.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

Rosmarinus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMTG8-Amp-FRT

Plasmid

#128277

Purpose

TIP-LITer2.0-KRAS(G12V) gene circuit with the following architecture: CMV-TetOx2-TetR-LOV-TIP-P2A-KRAS(G12V)-P2AGFP

Depositor

Gabor Balazsi

Article

Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

Inserts

TetR-LOV-TIP
EGFP
KRAS(G12V)

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEM01

Plasmid

#135414

Purpose

Expression of Hygromycin resistance from CMV promoter

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

CMVpr hph ADH1t

Expression

Bacterial and Yeast

Promoter

CMVpr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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OccludinMut_Flag

Plasmid

#86043

Purpose

Full length Occludin with mutation of PPPY domain at AA9-12 to PAAY

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2010 Aug;299

Insert

OccludinMut (OCLN Human)

Tags

FLAG

Expression

Mammalian

Mutation

PPPY domain at AA9-12 to PAAY; I52T and Q365P

Promoter

CMV

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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p3x-Flag Gli3 37R3-2

Plasmid

#120305

Purpose

Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37C

Depositor

David Liu

Article

Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi

Insert

Intein 37R3-2, Gli3

Use

Synthetic Biology

Tags

3X Flag

Expression

Mammalian

Mutation

Contains mutations as reported in 2011 Chemistry …

Promoter

CMV

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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hRad51(G151D)–Cas9(D10A)

Plasmid

#125565

Purpose

Expresses the Rad51(G151D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(G151D)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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hRad51(F86E)–Cas9(D10A)

Plasmid

#125564

Purpose

Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(F86E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAB-NEO-PYL

Plasmid

#114393

Purpose

For PYL expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL

Tags

3xFLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-M4-AR-W741C-S81A-P82A

Plasmid

#171250

Purpose

Mammalian expression of 3xFlag-tagged human androgen receptor Serine-Proline mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82A)

Depositor

Steven Balk

Insert

3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala-Pro82Ala mutant (AR Human)

Tags

Flag

Expression

Mammalian

Mutation

AR-Trp741Cys-Ser81Ala-Pro82Ala (AR-W741C-S81A-P82…

Promoter

CMV

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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