We narrowed to 23,671 results for: CRISPR

1. pBS-GMR-eya(shRNA)

   Plasmid

   #157991

   Purpose

   pBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.

   Depositor

   Greg Beitel

   Article

   Nyberg et al Fly (Austin). 2020 Oct 5. doi: 10.1080/1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-U6-empty

   Plasmid

   #140580

   Purpose

   Expresses a gRNA and mCherry in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. DS-SPcas

   Plasmid

   #48645

   Purpose

   Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Inserts

   Cas9
   tracrRNA precursor
   

   Use

   CRISPR

   Promoter

   proC and tracrRNA promoter

   Available Since

   Dec. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. p206_LTJ_sgRNACD45.2_R3

   Plasmid

   #82674

   Purpose

   sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

   Depositor

   Lukas Jeker

   Article

   Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

   Insert

   sgRNA targeting CD45.2 region 3
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFD5-NS

   Plasmid

   #149546

   Purpose

   Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Promoter

   U6

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pG2

   Plasmid

   #162603

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Expression

   Yeast

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pG4

   Plasmid

   #162605

   Purpose

   Edit Ade2 gene in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Expression

   Yeast

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB3405

   Plasmid

   #106166

   Purpose

   EasyCloneYALI system-based yeast episomal vector carrying a nourseothricin-resistance marker for Yarrowia lipolytica, can be used for gRNA expression, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9m4

   Plasmid

   #47316

   Purpose

   Cas9 D10A+D839A+H840A+N863A

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m4
   

   Use

   CRISPR

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCR-U6-gRNA-miniCMV-TdTomato

    Plasmid

    #192656

    Purpose

    A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    TdTomato
    

    Expression

    Mammalian

    Promoter

    U6, miniCMV

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 6xHis-MBP-huErCas12a

    Plasmid

    #174685

    Purpose

    Expresses 6xHis-MBP tagged ErCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huErCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis

    Expression

    Bacterial

    Mutation

    Q926R mutation is observed as indicated from the …

    Promoter

    T7

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIKU801-CrPEPC1

    Plasmid

    #92342

    Purpose

    Expresses sgRNA for targeting CrPEPC1 gene, paromomycin resistance

    Depositor

    I-Son Ng

    Article

    Kao et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    sgRNA-CrPEPC1
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU6-26

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. M-NM-sgRNA

    Plasmid

    #48673

    Purpose

    Mammalian U6-driven sgRNA (NMm1) targeting GTCCCCTCCACCCCACAGTG

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with N. meningitidis Cas9, hU6 promoter
    

    Use

    CRISPR

    Promoter

    hU6

    Available Since

    Oct. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL193

    Plasmid

    #180471

    Purpose

    For expression of Cas9 and gRNAs, multiple gRNAs cloning vector

    Depositor

    Peggy Lemaux

    Article

    Aregawi et al Plant Biotechnol J. 2021 Nov 26. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Cas9

    Expression

    Plant

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEX128·gRNA

    Plasmid

    #187600

    Purpose

    Template for amplification of gRNA with Cas6 recognition site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    gRNA template
    

    Promoter

    no promoter

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116954

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKM197

    Plasmid

    #132665

    Purpose

    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.

    Depositor

    Joseph Sorg

    Article

    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:

    Insert

    Upstream & Downstream pyrE deletion region
    

    Use

    E. coli - c. difficile shuttle vector

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-Stuffer

    Plasmid

    #106248

    Purpose

    Destination vector for U6::gRNA expression cassette

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Type

    Empty backbone

    Use

    AAV

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMM704

    Plasmid

    #68891

    Purpose

    IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p458 VQR

    Plasmid

    #101727

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Expression

    Mammalian

    Mutation

    VQR

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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