We narrowed to 15,981 results for: LEA

1. pAG415Gal_Gcn4 ILVtoED

   Plasmid

   #232957

   Purpose

   Galactose iduced expression of Gcn4 ILVtoED in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   L123D, I128E, V130E, V135E, L137D, I142D

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG415Gal_Gcn4 StoL

   Plasmid

   #232981

   Purpose

   Galactose iduced expression of Gcn4 StoL in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 StoL
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S104L, S117L, S136L, S144L

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG415Gal_Gcn4 R3W

   Plasmid

   #232975

   Purpose

   Galactose iduced expression of Gcn4 R++ W+ in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 R++ W+
   

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   D103R, N126W, D133R, K143R

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG415Gal_Gcn4 E3HA

   Plasmid

   #232996

   Purpose

   Galactose iduced expression of Gcn4 E+HA in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 E+
   

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   D105E, D118E, D139E

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG415Gal_Gcn4 F108A+

   Plasmid

   #232974

   Purpose

   Galactose iduced expression of Gcn4 F108A in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 F108A
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   F108A, T121L, A141P

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG415Gal_Gcn4 ILVtoAS

   Plasmid

   #232959

   Purpose

   Galactose iduced expression of Gcn4 ILVtoAS in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoAS
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   L123S, I128S, V130A, V135A, L137S, I142S

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest17_Gcn4 S/ArotoL

   Plasmid

   #231880

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 SArotoL

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 S/ArotoL
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101L, S104L, F108L, Y110L, S117L, S136L, S144L

   Promoter

   T7

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest17_Gcn4 solvvol W

   Plasmid

   #231878

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol W
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   T7

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDest17_Gcn4 solvvol

   Plasmid

   #231877

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   T7

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDest17_Gcn4 9acidA

    Plasmid

    #231867

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 9acidA

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 9acidA
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

    Promoter

    T7

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. JDW 1035 (pME_V5_TCF7L1_P2A_H2A_mCherry)

    Plasmid

    #232125

    Purpose

    A middle entry gateway clone containing V5 tagged human TCFL7 / TCF3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    TCF7L1 / TCF3 (TCF7L1 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDest17_Gcn4 WTSLFD

    Plasmid

    #231881

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 WTSLFD

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 WTSLFD
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    F108W, E109T, Y110S, E111L, N112F, L113D

    Promoter

    T7

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDest17_Gcn4 ILVtoED W

    Plasmid

    #231871

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED W

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED W
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    N126W, I128E, V130E, V135E, L137D, I142D

    Promoter

    T7

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDest17_Gcn4 ILVtoAS

    Plasmid

    #231859

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoAS

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoAS
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    L123S, I128S, V130A, V135A, L137S, I142S

    Promoter

    T7

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDest17_Gcn4 ILVtoED

    Plasmid

    #231857

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 ILVtoED

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 ILVtoED
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    L123D, I128E, V130E, V135E, L137D, I142D

    Promoter

    T7

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTYB1-T4gp47

    Plasmid

    #228212

    Purpose

    Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

    Depositor

    Stephen Benkovic

    Article

    Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

    Insert

    47 (47 Escherichia phage T4)
    

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-hfYFP-TEV-mScarlet-i

    Plasmid

    #191196

    Purpose

    Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & mScarlet-I fusion
    

    Tags

    6xHis and mScarlet-I

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-LSSmGFP-TEV-mScarlet-i

    Plasmid

    #191199

    Purpose

    Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP & mScarlet-I fusion
    

    Tags

    6xHis and mScarlet-I

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. R152C USP7 TRAF

    Plasmid

    #227229

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    R152C; aa 62-205

    Promoter

    T7-promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. G392A FL USP7

    Plasmid

    #227217

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392A

    Promoter

    T7-promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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