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pHR_UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

Plasmid

#188770

Purpose

dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

dCas9-XTEN80-KRAB(Kox1)

Use

Lentiviral

Tags

HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

Promoter

SFFV

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-CasE-p300-Flag

Plasmid

#106275

Purpose

Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

EcoCasE-p300

Use

CRISPR

Tags

E tag, NLS and Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Xpress beta4 1355T

Plasmid

#16040

Depositor

Filippo Giancotti

Article

Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

Insert

beta4 (ITGB4 Human)

Tags

6xHis and Xpress

Expression

Mammalian

Mutation

1355T: amino acids 1-1355

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_SNORD27h_2ntmut

Plasmid

#73067

Purpose

expression clone for human mutated SNORD27 (C/D box snoRNA U27)

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

SNORD27 (E2F7 Human)

Tags

no tag

Expression

Mammalian

Mutation

2 nt mutation in AS box that binds E2F7 pre-mRNA

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pLKO.1-shMBNL3-0455

Plasmid

#115463

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-0455 (MBNL3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4D-CD4-FLAG

Plasmid

#51601

Purpose

expresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D/CD4-FLAG (Sema4d Mouse, Human)

Tags

FLAG and myc

Expression

Mammalian

Mutation

Sema4D amino acids 660-861 were replaced with CD4…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_hE2F7mg_PP7-tag

Plasmid

#73078

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-end

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Tags

5'-PP7-tag RNA

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-cAICDA-BE3

Plasmid

#113422

Purpose

Expresses cAICDA-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

cAICDA-BE3 (aicda Ictalurus punctatus (channel catfish))

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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PWPXLD-GFP-Numb-S276D

Plasmid

#133752

Purpose

Numb with mutation at serine 276 converted to aspartic acid cand tagged with GFP cloned into PWPXLD

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

NUMB endocytic adaptor protein (Numb Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

serine 276 converted to aspartic acid

Promoter

EF1-alpha

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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FRT1-His-H2B-Cherry-2A (IG156)

Plasmid

#99622

Purpose

To clone gene of interest downstream of FRT1-His-H2B-Cherry-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

His-H2B-Cherry

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shMBNL3-9465

Plasmid

#115464

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shMBNL3-9465 (MBNL3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-AURKB 3'UTR

Plasmid

#29475

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

AURKB 3"UTR (AURKB Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pCR3-vsv-AuroraB L154G/H250Y

Plasmid

#108490

Purpose

expression of vsv-AuroraB Analog sensitive (L154G/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

VSV

Expression

Mammalian

Mutation

Analog sensitive L154G/H250Y

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hE2F7mg_2ntmut

Plasmid

#73074

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns, 2…

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
FUW TetO NET1A-V5 S46A

Plasmid

#69829

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

S46A

Promoter

minimal CMV promoter with tetracycline operator

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 E17K;K177M

Plasmid

#73049

Purpose

Human AKT3 E17K;K177M

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 E17K;K177M (AKT3 Human)

Expression

Mammalian

Mutation

E17K;K177M

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pRRL_LYSET_Y34X

Plasmid

#246084

Purpose

Stable expression of LYSET Y34X (isoform 2; Y72X in isoform 1)

Depositor

Wilhelm Palm

Article

Pechincha et al Science. 2022 Oct 7;378(6615):eabn5637.

Insert

LYSET Y34X (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL_LYSET

Plasmid

#246082

Purpose

Stable expression of LYSET (isoform 2)

Depositor

Wilhelm Palm

Article

Pechincha et al Science. 2022 Oct 7;378(6615):eabn5637.

Insert

LYSET (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL_LYSET_R7W

Plasmid

#246083

Purpose

Stable expression of LYSET R7W (isoform 2; R45W in isoform 1)

Depositor

Wilhelm Palm

Article

Pechincha et al Science. 2022 Oct 7;378(6615):eabn5637.

Insert

LYSET R7W (LYSET Human)

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_G-PTEN

Plasmid

#227432

Purpose

Expresses the G-PTEN sensor under a CMV promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN (Pten Rat)

Tags

mEGFP and sREACh

Expression

Mammalian

Mutation

R14G

Promoter

CMV

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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