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Mirror RFP[ENE(WT)-mascRNA](pAVA2987)

Plasmid

#239348

Purpose

Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of wild-type MALAT1 ENE-mascRNA reporter.

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

MALAT1 ENE(WT)-mascRNA

Expression

Mammalian

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

Plasmid

#238286

Purpose

For overexpression of mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG

Plasmid

#238283

Purpose

For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPGrp

Plasmid

#236002

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to lysosome surface.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Lyso-InPGrp (Cyth3 Synthetic, Rat)

Tags

Cerulean, Full-length LAMP1, and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX.(Cyto)iGlucoSnFR.H66A.L276V-mRuby2

Plasmid

#238943

Purpose

Green glucose sensor with mRuby2 fusion, high sensitivity, cytosolic

Depositor

Paul Khavari

Article

Lopez-Pajares et al Cell Stem Cell. 2025 Mar 20:S1934-5909(2

Insert

iGlucoSnFR.L276V.K312A

Use

Lentiviral

Tags

Myc-mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS

Plasmid

#237662

Purpose

For integration of EGFP-NPM1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_EGFP-NPM1-KS-0F

Plasmid

#237665

Purpose

For integration of EGFP-NPM1-KS-0F

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS-0F (NPM1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-NPM1-KS

Plasmid

#237668

Purpose

For overexpression of mEGFP-NPM1-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1-KS (NPM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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EW378 (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-GFP

Plasmid

#236134

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with four ZNF35/ZNF250 bindi…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW411 (UTRN-43-5bp spacer-UTRN-14)-H2B-GFP

Plasmid

#236148

Purpose

Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the UTRN promoter region encompassing 14 to 67 bp upstream of its transcriptional start site

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with the UTRN promoter region

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW407 (ZNF35/ZNF35-6bp spacers)x4-H2B-GFP

Plasmid

#236137

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNF250 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with four ZNF35/ZNF250 bindi…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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SCN1Aprom(335-406)-H2B-GFP

Plasmid

#236151

Purpose

Fluorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with the SCN1a promoter region encompassing 335 to 406 bp upstream of its transcriptional start site

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with the SCN1a promoter regi…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW387 (ZNF35/IKZF1tar-6bp spacers)x4-H2B-GFP

Plasmid

#236138

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with four ZNF35/ZNFIKZF1 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al Cell Syst. 2025 Jul 16;16(7):101299. doi

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with four ZNF35/ZNFIKZF1 bin…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (BB)-2A-GFP-RB1sg

Plasmid

#229857

Purpose

single guide RNA targeting human RB1 gene

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al FASEB J. 2025 Mar 15;39(5):e70431. doi:

Insert

RB1sgRNA (RB1 Human)

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pmNeon Green-N1-ARF3-R

Plasmid

#202431

Purpose

Mammalian expression vector containing pmNeonGreen-tagged ARF3

Depositor

David Bryant

Article

Sandilands et al J Cell Biol. 2023 Apr 3;222(4):e20220611

Insert

ARF3 (ARF3 Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAP

Plasmid

#222322

Purpose

Synchronize the trafficking of LAP from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAP fused to SBP-EGFP (ACP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-EGFR

Plasmid

#222325

Purpose

Synchronize the trafficking of EGFR from the ER.

Depositor

Franck Perez

Article

Scharaw et al J Cell Biol. 2016 Nov 21;215(4):543-558.

Insert

Streptavidin-KDEL and EGFR fused to SBP-EGFP (EGFR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_sgALDH7A1

Plasmid

#218273

Purpose

CRISPR KO plasmid for ALDH7A1 in human cell lines

Depositor

Seth Parker

Article

Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

Insert

Inserted sgRNA targeting ALDH7A1 (ALDH7A1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-GFP_TMEM88B

Plasmid

#227954

Purpose

Co-localisation experiment

Depositor

Alessandro Ori

Article

Ghoochani et al Cell. 2026 Feb 5;189(3):765-782.e31. doi

Insert

TMEM88B

Use

Lentiviral

Tags

3xFLAG-EGFP

Expression

Mammalian

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SAM-DNMT3A-inactive+sgGFP

Plasmid

#213169

Purpose

Vector with sgGFP used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

Depositor

Sefi Rosenbluh

Article

Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

Insert

sgGFP

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

E1Fa and U6

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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