We narrowed to 23,671 results for: CRISPR

1. pEcCas6

   Plasmid

   #170091

   Purpose

   expresses E. coli type I-E Cas6

   Depositor

   Chase Beisel

   Article

   Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

   Insert

   E. coli type I-E Cas6
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PM-SP!TB

   Plasmid

   #48650

   Purpose

   Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Bacterial SP crRNA to prototspacer B
   

   Use

   CRISPR

   Promoter

   J23100 promoter

   Available Since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3s-eCas9-1.1

   Plasmid

   #104172

   Purpose

   eCas9-1.1 mammalian expression

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

   Insert

   eCas9-1.1
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   CMV

   Available Since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmCherry-U6-EMX1

   Plasmid

   #140581

   Purpose

   Expresses a gRNA and mCherry in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   gRNA EMX1
   

   Use

   CRISPR

   Available Since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pARS3_MUbCAS9_MC

   Plasmid

   #128767

   Purpose

   T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS for cloning gRNA

   Depositor

   Zachary Nimchuk

   Article

   Worthen et al Development. 2019 Jun 3. pii: dev.174870

   Type

   Empty backbone

   Tags

   HA

   Expression

   Plant

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. px459 EQR

   Plasmid

   #101732

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Expression

   Mammalian

   Mutation

   VQR

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. XX>sgRNA F+E

   Plasmid

   #87543

   Purpose

   Template 2 (of 2) for U6>sgRNA cassette OSO-PCR

   Depositor

   Lionel Christiaen

   Article

   Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

   Type

   Empty backbone

   Available Since

   Jan. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. BCJJ358

   Plasmid

   #117505

   Purpose

   RPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverse

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtRPS5a

   Available Since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9m2-VP64

   Plasmid

   #47317

   Purpose

   Cas9m2 Activator

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m2-VP64
   

   Use

   CRISPR

   Available Since

   Aug. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

10. BCJJ344

    Plasmid

    #117503

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtUBI10

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2

    Plasmid

    #116953

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. library backbone 3'LTR gRNA and barcode

    Plasmid

    #127397

    Purpose

    Digestion by BstXI and BamHI to release the backbone

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Puro-mCherry

    Available Since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTFE3-donor

    Plasmid

    #112377

    Purpose

    CRISPR donor plasmid to tag human transcription factor TFE3 with GFP

    Depositor

    Kevin White

    Insert

    TFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
    

    Use

    CRISPR

    Mutation

    hg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. AeU6 3p3 GFP LgRNA

    Plasmid

    #182208

    Purpose

    For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    3xP3-GFP
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6

    Available Since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMLS544

    Plasmid

    #188882

    Purpose

    Integrating Pmex-5::Cas9 expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMulti-sgRNA-LacZ

    Plasmid

    #99913

    Purpose

    Receptor plasmid for the assembly of multiple sgRNAs

    Depositor

    Yujie Sun

    Article

    Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmCherry-U6-VEGFA

    Plasmid

    #140588

    Purpose

    Expresses a gRNA and mCherry in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    gRNA VEGFA
    

    Use

    CRISPR

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMP2367

    Plasmid

    #160076

    Purpose

    Z. mobilis CRISPRi, promoter C, empty guide, sfGFP

    Depositor

    Jason Peters

    Article

    Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

    Insert

    empty sgRNA
    

    Expression

    Bacterial

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMLS546

    Plasmid

    #188883

    Purpose

    Integrating Pmex-5::Cas9 expression vector

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Pmex-5::Cas9
    

    Expression

    Worm

    Mutation

    C. elegans codom optimization

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6Rosa-CAG-Cas9

    Plasmid

    #118998

    Purpose

    Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    Cas9
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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