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POC1355

Plasmid

#221572

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1343, POC1344, POC1345 and POC1346; respectively

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1430

Plasmid

#221577

Purpose

Assembly vector designed to be methylated by the M.Osp807II BsaI-associated switch methylase and for the assembly of inserts 1, 2, 3 and 4 from POC1426, POC1427, POC1428 and POC1429; respectively

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1518

Plasmid

#221579

Purpose

pMOBK360_VL: Vector Left (VL) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1519

Plasmid

#221580

Purpose

pMOBK360_VM: Vector Middle (VM) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS3-rab-3P::AI::QF+hGR

Plasmid

#232611

Purpose

Pan-neuronal expression of chimeric protein 'QF+hGR', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

QF+hGR

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::QF+hGR

Plasmid

#232611

Purpose

Pan-neuronal expression of chimeric protein 'QF+hGR', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

QF+hGR

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::CHRIMSON_CE

Plasmid

#232603

Purpose

Pan-neuronal expression of opsin 'Chrimson', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

Chrimson

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::CHRIMSON_CE

Plasmid

#232603

Purpose

Pan-neuronal expression of opsin 'Chrimson', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

Chrimson

Expression

Worm

Mutation

N/A

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::Voltron

Plasmid

#232602

Purpose

Pan-neuronal expression of voltage sensor 'Voltron', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

Voltron

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS3-rab-3P::AI::Voltron

Plasmid

#232602

Purpose

Pan-neuronal expression of voltage sensor 'Voltron', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

Voltron

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS3-rab-3P::AI::TsChR

Plasmid

#232604

Purpose

Pan-neuronal expression of opsin 'TsChR', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

TsChR

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAS3-rab-3P::AI::TsChR

Plasmid

#232604

Purpose

Pan-neuronal expression of opsin 'TsChR', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

TsChR

Expression

Worm

Mutation

N/A

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1468

Plasmid

#213756

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.BsaI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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POC1468

Plasmid

#213756

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.BsaI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1467

Plasmid

#213755

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.BsaI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
POC1467

Plasmid

#213755

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Non-switchable methylase M2.BsaI

Tags

His6-tag

Expression

Bacterial

Promoter

T7

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN012

Plasmid

#220051

Purpose

Reporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide (positive control).

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

GFP-fLuc

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAN012

Plasmid

#220051

Purpose

Reporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide (positive control).

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

GFP-fLuc

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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GB3683

Plasmid

#187808

Purpose

Transcriptional unit for expression of 1,2-diacyl-sn-glycerol:acetyl-CoA acetyltransferase from Euonymus fortunei, codon optimized for Nicotiana.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

EfDAct

Expression

Plant

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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GB3683

Plasmid

#187808

Purpose

Transcriptional unit for expression of 1,2-diacyl-sn-glycerol:acetyl-CoA acetyltransferase from Euonymus fortunei, codon optimized for Nicotiana.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

EfDAct

Expression

Plant

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge