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MAC-C Sars-CoV-2 S_D80A

Plasmid

#194834

Purpose

Sars-CoV-2 S_D80A in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

S_D80A (S Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

D80A

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 N_R203K+G204R

Plasmid

#194809

Purpose

Sars-CoV-2 N_R203K+G204R in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

N_R203K+G204R (N Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

R203K+G204R

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP13_E341D

Plasmid

#194814

Purpose

Sars-CoV-2 NSP13_E341D in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP13_E341D (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

E341D

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP13_P77L

Plasmid

#194815

Purpose

Sars-CoV-2 NSP13_P77L in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP13_P77L (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

P77L

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP2_T85I

Plasmid

#194816

Purpose

Sars-CoV-2 NSP2_T85I in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP2_T85I (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

T85I

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP3_A890D

Plasmid

#194817

Purpose

Sars-CoV-2 NSP3_A890D in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP3_A890D (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

A890D

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-C Sars-CoV-2 NSP3_K977Q

Plasmid

#194818

Purpose

Sars-CoV-2 NSP3_K977Q in MAC-C destination vector

Depositor

Markku Varjosalo

Article

Varjosalo et al 10 May 2024, Research Square

Insert

NSP3_K977Q (ORF1ab Sars-CoV-2)

Tags

MAC-C

Expression

Mammalian

Mutation

K977Q

Available Since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-enCas12a-A3A-N57G

Plasmid

#193651

Purpose

Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-hA3A-N57G, EGFP

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD

Plasmid

#196878

Purpose

Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2-N-gTBD

Use

Cre/Lox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD

Plasmid

#196881

Purpose

Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2-Cdk5rap2-CTD

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-Lamp1 FLAG

Plasmid

#199750

Purpose

Lentiviral construct to stably express Lamp1

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Lamp1 (Lamp1 Rat)

Tags

Flag

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-myc Metap2

Plasmid

#199685

Purpose

Transient expression of Metap2

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

METAP2 (METAP2 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-Sox2AV

Plasmid

#193354

Purpose

Retroviral vector expressing mouse Sox2A61V point mutant

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Sox2AV (Sox2 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Sox2A61V is a highly cooperative point mutant of …

Promoter

5'LTR

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2AV

Plasmid

#193352

Purpose

Retroviral vector expressing human SOX2A61V point mutant

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2AV (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

SOX2A61V is a highly cooperative point mutant of …

Promoter

5'LTR

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPrdm9#1/Cre

Plasmid

#193231

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Prdm9 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgPrdm9#1 (Prdm9 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgPrdm9#2/Cre

Plasmid

#193232

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Prdm9 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgPrdm9#2 (Prdm9 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgMroh2b#2/Cre

Plasmid

#193220

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Mroh2b gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgMroh2b#2 (Heatr7b2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgMroh2b#1/Cre

Plasmid

#193219

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Mroh2b gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgMroh2b#1 (Heatr7b2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIFEX-A2aGH

Plasmid

#160542

Purpose

pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEFEX1-A2aGH

Plasmid

#160543

Purpose

pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

Depositor

Michelle O'Malley

Article

Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

Insert

Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)

Expression

Yeast

Mutation

N/A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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