We narrowed to 15,824 results for: LEA

1. pCI-EGFP-NR1 wt

   Plasmid

   #45446

   Depositor

   Robert Malinow, Andres Barria

   Article

   Barria et al Neuron. 2002 Jul 18;35(2):345-53.

   Insert

   NR1 (Grin1 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EGFP inserted after the predicted signal peptide …

   Promoter

   CMV

   Available since

   June 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-SpyTag002-MBP

   Plasmid

   #102831

   Purpose

   Bacterial expression of SpyTag002 fused to maltose binding protein

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyTag002-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to the N-terminus of MBP via a …

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAR-Ec633

   Plasmid

   #130873

   Purpose

   A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (L477V, L640Y, I777K, W814N) for OrthoRep

   Depositor

   Chang Liu

   Article

   Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

   Insert

   TP-DNAP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   L477V, L640Y, I777K, W814N

   Promoter

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-BaLgp160

   Plasmid

   #100919

   Purpose

   Mammalian expression plasmid for Env from the BaL HIV-1 isolate

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BaL) Env
   

   Use

   Tags

   CD5 leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9 (PX165)

   Plasmid

   #48137

   Purpose

   Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB402SC

   Plasmid

   #224552

   Purpose

   Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.

   Depositor

   Paul Schulze-Lefert

   Article

   Lawson et al bioRxiv 2024.07.14.603419

   Type

   Empty backbone

   Use

   Tags

   Twin-Strep-HA

   Expression

   Plant

   Mutation

   Promoter

   2 X 35S

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB402SN

   Plasmid

   #224551

   Purpose

   Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

   Depositor

   Paul Schulze-Lefert

   Article

   Lawson et al bioRxiv 2024.07.14.603419

   Type

   Empty backbone

   Use

   Tags

   HA-Twin-Strep

   Expression

   Plant

   Mutation

   Promoter

   2 X 35S

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. cr3Dd

   Plasmid

   #184862

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, bet, exo
   

   Use

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRCC-N

   Plasmid

   #81192

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-DU422gp160

    Plasmid

    #100926

    Purpose

    Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (DU422.1) Env
    

    Use

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-CH111gp160

    Plasmid

    #123268

    Purpose

    Mammalian expression plasmid for Env from the CH111 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCEP4-B41gp160

    Plasmid

    #123247

    Purpose

    Mammalian expression plasmid for Env from the B41 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (B41) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMF-TetTight

    Plasmid

    #193288

    Purpose

    Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

    Depositor

    Rudolf Jaenisch

    Article

    Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

    Insert

    rtTA-P2A-tTS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF‐1α

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-AD8gp160

    Plasmid

    #123260

    Purpose

    Mammalian expression plasmid for Env from the AD8 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCEP4-25711gp160

    Plasmid

    #111841

    Purpose

    Mammalian expression plasmid for Env from the 25711 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (25711.2) Env
    

    Use

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCEP4-CH115gp160

    Plasmid

    #123280

    Purpose

    Mammalian expression plasmid for Env from the CH115 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (CH115) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-H2B-GFP

    Plasmid

    #116869

    Purpose

    Nuclear localized GFP

    Depositor

    Loren Looger

    Article

    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-001428gp160

    Plasmid

    #123255

    Purpose

    Mammalian expression plasmid for Env from the 001428 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (001428) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCEP4-H031gp160

    Plasmid

    #123272

    Purpose

    Mammalian expression plasmid for Env from the H031 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (H031) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JDW 1039 (pLenti6.2_CMV_V5_TCF7_P2A_H2A_mCherry)

    Plasmid

    #233539

    Purpose

    A CMV driven human TCF7 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    TCF7 (TCF7 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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