We narrowed to 90,102 results for: Mal

1. iMb2-Control-Mosaic (SO273)

   Plasmid

   #99750

   Purpose

   Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins

   Depositor

   Rui Benedito

   Article

   Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

   Insert

   Mb2YFP-2A, Mb2Tomato-2A, Mb2-HA-Tfp1
   

   Use

   Cre/Lox and Mouse Targeting

   Expression

   Mammalian

   Available Since

   Oct. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. retro-gfp-puro vector

   Plasmid

   #58249

   Purpose

   Retroviral expression vector encoding bicistronic EGFP and Puromycin cassette

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

   Insert

   EGFP
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV Puro DHFR.dn-cHSF1

   Plasmid

   #134729

   Purpose

   Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain

   Depositor

   Matthew D. Shoulders

   Article

   Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

   Insert

   DHFR.dn-cHSF1 (HSF1 Human)
   

   Use

   Lentiviral

   Tags

   DHFR

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 186-202, 379−529

   Promoter

   CMV

   Available Since

   Aug. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLNCX2-NGN2-IRES-GFP-T2A-Sox11

   Plasmid

   #90213

   Purpose

   To convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

   Insert

   NGN2-IRES-GFP-T2A-Sox11 (Sox11, NEUROG2 Mouse, Human)
   

   Use

   Retroviral

   Promoter

   CMV

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pODN-mR2-ACTB

   Plasmid

   #183868

   Purpose

   Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   ACTB homology arms with mRuby2-linker (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-linker

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TetO-dCas9-mD3A

   Plasmid

   #78255

   Purpose

   Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) linked to IRES-mCherry under the control of TetOp and a minimal CMV promoter

   Depositor

   Grant Challen

   Article

   McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

   Insert

   DNMT3A CD aa 598-912 (DNMT3A Human)
   

   Use

   CRISPR

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Mutation

   E756A

   Promoter

   CMV

   Available Since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEx1-pEF-H2B-mCherry-T2A-rTetR-HDAC4-Zeo

   Plasmid

   #78353

   Purpose

   Expresses rTetR-HDAC4 for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

   Depositor

   Michael Elowitz

   Article

   Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

   Insert

   H2B-mCherry-T2A-rTetR-HDAC4 (HDAC4 Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   rTetR

   Expression

   Mammalian

   Promoter

   pEF alpha

   Available Since

   July 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBCAG-rtTAM2-2A-SOX17-GR-IH

   Plasmid

   #165079

   Purpose

   Ubiquitously expresses rtTAM2, dexamethasone-inducible human SOX17, and hygromycin-resistance gene.

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

   Inserts

   rtTA-M2
   SOX17 (SOX17 Human)
   

   Use

   Piggybac transposition

   Tags

   Glucocorticoid Receptor

   Expression

   Mammalian

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBbi-RP-CoV2/stSpike-puro

   Plasmid

   #161794

   Purpose

   Transposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein production

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

   Insert

   SARS-CoV-2 secretable trimeric Spike
   

   Use

   Transposon

   Tags

   6xHis and dTomato

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX LYCHOS (GPR155) WT-FLAG

    Plasmid

    #199633

    Purpose

    Lentiviral construct to stably express LYCHOS (GPR155) WT

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    GPR155 (GPR155 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLgw EcoDam-V5-ELK1

    Plasmid

    #98604

    Purpose

    Mammalian DamID lentiviral vector for ELK1 with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK1 (Elk1 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1

    Plasmid

    #196890

    Purpose

    Neuron-specific expression of human (h)Centrin fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mNeonGreen-hCentrin1 (CETN1 Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXPR_sgFLNB-SK2

    Plasmid

    #119873

    Purpose

    gRNA expression vector

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    sgFLNB-SK2 (FLNB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXPR_sgFLNB-SK4

    Plasmid

    #119874

    Purpose

    gRNA expression vector

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    sgFLNB-SK4 (FLNB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293

    Plasmid

    #118412

    Purpose

    Expresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.

    Depositor

    Nicole Paulk

    Article

    Rumachik et al Mol Ther Methods Clin Dev. 2020 May 22;1

    Insert

    Firefly luciferase
    

    Use

    AAV, Luciferase, and Synthetic Biology

    Expression

    Insect and Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

    Plasmid

    #198569

    Purpose

    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    changed glutamic acid E164 to lysine (E164K)

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFPc1 hp47phox (S303/304/328D)

    Plasmid

    #112245

    Purpose

    expression of mutant p47phox

    Depositor

    Simone Di Giovanni

    Article

    Hervera et al Nat Cell Biol. 2018 Mar;20(3):307-319. d

    Insert

    p47phox (NCF1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S303/304/328D

    Promoter

    CMV

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139097

    Purpose

    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    KRAB-dxCas9-NG
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-sgHcn1#2/Cre

    Plasmid

    #193218

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Hcn1 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgHcn1#2 (Hcn1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMD9_2xUGI_3xHA

    Plasmid

    #136652

    Purpose

    Expresses St1BE4max-LMD9 in mammalian cells along with its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    

    Use

    CRISPR and Synthetic Biology

    Tags

    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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