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pXD80ENSF5-Elen0515-eCadh-Accs(ElA2)

Plasmid

#225300

Purpose

Expresses Eggerthella lenta A2 eCadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

eCadh

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN2-Elen0501-(+)-Eddh-noAccs(ElA2)

Plasmid

#225310

Purpose

Expresses Eggerthella lenta A2 (+)-Eddh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eddh

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF18-C1878_02750_GsVadh(Gs28C)

Plasmid

#225304

Purpose

Expresses Gordonibacter sp. 28C Gs Vadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Gs Vadh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN3-Elen0507-Cadh-noAccs(ElA2)

Plasmid

#225311

Purpose

Expresses Eggerthella lenta A2 Cadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cadh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF3-Elen0507-Cadh-Accs(ElA2)

Plasmid

#225298

Purpose

Expresses Eggerthella lenta A2 Cadh in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Cadh

Expression

Bacterial

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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FAM177A1-Halo

Plasmid

#224591

Purpose

For expression of FAM177A1-Halo in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Tags

HALO

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA-MS2

Plasmid

#207624

Purpose

Expresses crRNA-MS2 in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

crRNA-MS2

Use

CRISPR

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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FAM177A1-SNAP

Plasmid

#224592

Purpose

For expression of FAM177A1-SNAPTag in mammalian cells

Depositor

Pietro De Camilli

Article

Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

Insert

FAM177A1 (FAM177A1 Human)

Tags

SNAP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp47

Plasmid

#228212

Purpose

Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

47 (47 Escherichia phage T4)

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BirA-HA3-HIS3MX6

Plasmid

#227975

Purpose

To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BioID2-HA3-HIS3MX6

Plasmid

#227977

Purpose

To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID3

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-BASU-HA3-HIS3MX

Plasmid

#227979

Purpose

To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, HIS3MX marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-PEX6-Tomato

Plasmid

#183730

Purpose

Expression of human PEX6 fused to Tomato, and PEX6 3'UTR

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Insert

PEX6-Tomato-PEX63'UTR

Use

Lentiviral

Promoter

UbiC

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1426

Plasmid

#218622

Purpose

Express tNCS to the peroxisome for toxicity assay

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

tNCS

Tags

YFP-ePTS1

Expression

Yeast

Mutation

106-588

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB1734

Plasmid

#218627

Purpose

Express amorphadiene synthase (ADS)

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

ADS

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB207

Plasmid

#218596

Purpose

Express degron assay: Degradation tag-YFP-ePTS1

Depositor

John Dueber

Article

Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

Insert

YFP

Tags

UbiY degradation tag and ePTS1

Expression

Yeast

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSliceRep_B_miR-196a.M3

Plasmid

#220471

Purpose

Dual luciferase reporter for RNAi, with target site within a tunable ORF (version B)

Depositor

David Bartel

Article

Wang et al Mol Cell. 2024 Jul 10:S1097-2765(24)0053

Insert

SUMO_Eu1

Tags

3xHA

Expression

Mammalian

Promoter

Minimal CMV

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-SMN2-inclusion-GLuc

Plasmid

#218671

Purpose

Alternative splicing signaling: signal increased with Ex7 inclusion increasing

Depositor

Jingxin Wang

Article

Tang et al Chembiochem. 2022 May 4;23(9):e202200012

Insert

SMN2 (SMN2 )

Expression

Mammalian

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pIA036

Plasmid

#200425

Purpose

amyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

Depositor

Rut Carballido-López

Article

Altinoglu et al Microbiol Spectr. 2024 Nov 4;12(12):e016

Insert

LacI

Tags

bsdronPA

Expression

Bacterial

Promoter

Ppen

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCfB8709

Plasmid

#175224

Purpose

Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

Depositor

Irina Borodina

Article

Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

Insert

YOL092W (CEN.PK strain)

Use

Mrna expression, injection into xenopus oocytes

Tags

PolyA

Promoter

T7

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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