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pICSL86977OD:PITG_15278a

Plasmid

#173545

Purpose

This is a binary plasmid that can be transferred into Agrobacterium and used to transiently express the protein encoded by the insert in plants by agro-infiltration.

Depositor

Sophien Kamoun

Article

Derevnina et al PLoS Biol. 2021 Aug 23;19(8):e3001136. d

Insert

PITG_15278_P17777

Expression

Plant

Promoter

35S

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:S495A

Plasmid

#98151

Purpose

Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:S495A

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed serine 495 to alanine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:W603A

Plasmid

#98153

Purpose

Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:W603A

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed tryptophan 603 to alanine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:H695Q

Plasmid

#98154

Purpose

Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:H695Q

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed histidine 695 to glutamine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:H695A

Plasmid

#98155

Purpose

Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:H695A

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed histidine 695 to alanine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:Y696G

Plasmid

#98156

Purpose

Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:Y696G

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed tyrosine 696 to glycine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmEDC3_441-680_D

Plasmid

#146127

Purpose

Insect Expression of DmEDC3_441-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3_441-680 (Edc3 Fly)

Expression

Insect

Mutation

3 mutations N162T, K221E and D418G compared to th…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3_441-680_D

Plasmid

#146130

Purpose

Insect Expression of DmEDC3_441-680

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3_441-680 (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-D61A_D

Plasmid

#146131

Purpose

Insect Expression of DmEDC3-D61A

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3-D61A (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmEDC3-D61K_D

Plasmid

#146132

Purpose

Insect Expression of DmEDC3-D61K

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmEDC3-D61K (Edc3 Fly)

Expression

Insect

Mutation

one silent mutation A456G and three mutations N16…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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