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Plasmid#162557PurposeFor the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1DepositorInsert3FLAG-Mnase-SV40
UseTagsExpressionYeastMutationPromoterActineAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPL5628_TEF1*-Cre_MET15
Plasmid#60933PurposeConstitutive expression vector for Cre-recombinase containing MET15 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
ERAP1
Plasmid#39174PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertERAP1 (ERAP1 Human)
UseBaculovirus expressionTagsTEV-His10-FlagExpressionMutationPromoterpolyhedrinAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pADtet-His6-MscL
Plasmid#83373PurposeExpresses wild type MscL in mammalian cellsDepositorInsertMechanosensitive channel of large conductance (mscL E. coli)
UseAdenoviralTagsHis6 tagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-SAT1
Plasmid#162465PurposepFA-MNase-CaURA3 was double digested to remove the URA3 auxotrophy marker. The SAT1 marker was amplified from pFA-SAT1 and cloned into the AscI-SacI-digested pFA-MNaseDepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pMGE-T7
Plasmid#153021PurposeSingle-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pRK5_tAKARα-T391A
Plasmid#119920PurposeMammalian expression of the tAKARα sensor with T391A mutationDepositorInserttAKARα-T391A
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaARG4
Plasmid#162463PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaARG4DepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Cer.zf1
Plasmid#61389PurposemRNA synthesis of cerulean codon modified for zebrafishDepositorInsertCer.zf1
UseZebrafish expressionTagsExpressionMutationPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTAPmyc-T2A-tagRFP
Plasmid#140278PurposeBackbone for making a mammalian expression vector of the protein of interestDepositorInsertEmpty backbone
UseSynthetic BiologyTagsmyc-T2A-tagRFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNeurog2-Luciferase reporter
Plasmid#64126PurposePhotoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.DepositorInsertNeurog2 promoter
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterNeurog2Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS315Nab3
Plasmid#110532PurposeExpresses yeast NAB3 using the endogenous promoterDepositorInsertNAB3 (NAB3 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cip-pAct1-Mnase
Plasmid#162556PurposeNheI-MluI-digested 3xFLAG-MNase-SV40 fragment was cloned into the CIp-pACT1-CYC vector to ensure constitutive expression of MNase in C. albicans.DepositorInsert3FLAG-Mnase-SV40
UseTagsExpressionYeastMutationPromoterActineAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaURA3
Plasmid#162461PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaURA3DepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-MNase-CaHIS1
Plasmid#162462PurposeDNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-Ca HIS1DepositorInsert3FLAG-Mnase
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCsy-crRNA-EV
Plasmid#153944PurposeFor cloning and expression of Type I-F P. aeruginosa (PaeCascade) crRNA in mammalian cellsDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3 (in pCDNA3.1)
Plasmid#118041PurposeGABAA receptor expression (gamma3 subunit) Myc-tag near N-terminusDepositorInsertGABRG3 (Gabrg3 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma1 (in pCDNA3.1)
Plasmid#118038PurposeGABAA receptor expression (gamma1 subunit) Myc-tag near N-terminusDepositorInsertGABRG1 (Gabrg1 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only