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PX330-PTEN

Plasmid

#227440

Purpose

Cas9 expression and a gRNA targeting Exon 6 of mouse PTEN

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

spCas9 and gRNA for mouse PTEN (Pten )

Expression

Mammalian

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_G-PTEN_4A

Plasmid

#227434

Purpose

Expresses the G-PTEN sensor with a 4A mutation

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN 4A (Pten Rat)

Tags

mEGFP and sREACh

Expression

Mammalian

Mutation

4A mutation.

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCpep-E1GFP

Plasmid

#233884

Purpose

Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

Proinsulin-E1GFP (Ins2 Mouse)

Expression

Mammalian

Mutation

Inserted E1GFP into C-peptide of mouse Ins2 codin…

Promoter

CMVF

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pScalps-Puro-FLAG_HA-Regnase-1 D141N

Plasmid

#222663

Purpose

Lentiviral vector to express mouse Regnase-1 D141N mutant tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

SFFV

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA LTB4R

Plasmid

#226810

Purpose

Tet-inducible lentiviral vector for 2xHA LTB4R expression

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

LTB4R (LTB4R Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_858043.1

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA GPR153

Plasmid

#226808

Purpose

Gateway entry vector for 2xHA GPR153

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR153 (GPR153 Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_997253.2

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA LTB4R

Plasmid

#226806

Purpose

Gateway entry vector for 2xHA LTB4R

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

LTB4R (LTB4R Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_858043.1

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20 2xHA GPR137

Plasmid

#226813

Purpose

Tet-inducible lentiviral vector for 2xHA GPR137 expression

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR137 (GPR137 Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_001164351

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroQ-GTSE1-acGFP-S91, 262, 454, 724A

Plasmid

#224741

Purpose

Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Retroviral

Tags

acGFP

Mutation

Serine 91, 262, 454, 724 to Alanine

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroQ-GTSE1-acGFP-S91, 262, 454, 724D

Plasmid

#224742

Purpose

Retroviral vector for delivery and expression of acGFP1-tagged GTSE1 protein with S91, 262, 454, 724D mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Retroviral

Tags

acGFP

Mutation

Serine 91, 262, 454, 724 Aspartate; Valine 53 Iso…

Promoter

CMV

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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