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pLLK007

Plasmid

#245126

Purpose

Control plasmid based on pAF259 (Addgene #105494). Contains a promoterless luciferase-encoding bitluc.

Depositor

Robert Fagan

Article

Fisher et al Microbiology (Reading). 2026 Feb;172(2).

Insert

Bitlucopt

Expression

Bacterial

Promoter

None

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pANC61(FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron-IRES-EGFP)

Plasmid

#248354

Purpose

Lentiviral vector that can express hAqp1_degron based sensor for TEVp in mammalian cells. Expressed off of EF1a promoter with EGFP marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron

Use

Lentiviral

Tags

3x TEV site, FKBP12 degron and FLAG (internal in …

Promoter

EF-1a

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_cpCys203

Plasmid

#247771

Purpose

bacterial expression of cpCys203 FRET-based cysteine sensor

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

cpCys203 Cysteine Sensor

Tags

ECFP and Venus and His6-TEV

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-24

Plasmid

#246307

Purpose

Evaluation of a synthetic Pol III promoter based on the MtU6.6 promoter for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

MtU6.6m4 promoter

Use

CRISPR

Expression

Plant

Promoter

MtU6.6m4 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-25

Plasmid

#246308

Purpose

Evaluation of a synthetic Pol III promoter based on the MtU6.6 promoter for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

MtU6.6m5 promoter

Use

CRISPR

Expression

Plant

Promoter

MtU6.6m5 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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p305N-23

Plasmid

#246306

Purpose

Evaluation of a synthetic Pol III promoter based on the MtU6.6 promoter for CRISPR-Cas9 editing of mEGFP in a Nicotiana benthamiana reporter line

Depositor

Chung-Jui Tsai

Article

Deguchi et al Plant Physiol. 2025 Jul 3;198(3):kiaf294

Insert

MtU6.6m1 promoter

Use

CRISPR

Expression

Plant

Promoter

MtU6.6m1 promoter

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-S-13xLexAop-GCaMP8m_VK05

Plasmid

#238557

Purpose

A LexAop-based SPARC plasmid for SPARC-L. Can also be used as a standalone sparsener of a LexA driver line. Sparse (S) density. The GCaMP ORF can be swapped for your gene of interest.

Depositor

Tom Clandinin

Article

Currier et al Cell. 2025 Aug 7;188(16):4366-4381.e14.

Insert

GCaMP8m

Use

Sparc plasmid

Tags

DsRed-Express

Expression

Insect

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-I-13xLexAop-GCaMP8m_VK05

Plasmid

#238556

Purpose

A LexAop-based SPARC plasmid for SPARC-L. Can also be used as a standalone sparsener of a LexA driver line. Intermediate (I) density. The GCaMP ORF can be swapped for your gene of interest.

Depositor

Tom Clandinin

Article

Currier et al Cell. 2025 Aug 7;188(16):4366-4381.e14.

Insert

GCaMP8m

Use

Sparc plasmid

Tags

DsRed-Express

Expression

Insect

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-rev(CMV-TagBFP2)-EFS-mCherry-133-MRE

Plasmid

#235157

Purpose

Expresses the mCherry-based sensor for miR-133. TagBFP2 is expressed as a transduction reporter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mCherry with a miR-133 MRE from Ankrd28

Use

AAV

Promoter

EFS

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-rev(CMV-TagBFP2)-EFS-mCherry-124-WT-MRE

Plasmid

#235147

Purpose

Expresses the mCherry-based sensor for miR-124. TagBFP2 is expressed as a transduction reporter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mCherry with a miR-124 MRE from Slc31a2

Use

AAV

Promoter

EFS

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-rev(CMV-TagBFP2)-EFS-mCherry-137-MRE

Plasmid

#235160

Purpose

Expresses the mCherry-based sensor for miR-137. TagBFP2 is expressed as a transduction reporter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mCherry with a miR-137 MRE from Neurod4

Use

AAV

Promoter

EFS

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-luxNeon

Plasmid

#229942

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxNeon

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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RT-2

Plasmid

#220438

Purpose

Base construct used to create PUP2-DADA/3HA fusion proteins in S. cerevisiae. (aka pBSII/PUP2-DADA/3HA/URA3)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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Modular BE4max for GG insertion

Plasmid

#198891

Purpose

Easily insert deaminase of choice into BE4max using Golden Gate cloning

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

CRISPR associated protein 9 (cas9 Streptococcus pyogenes)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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ppih.001.177.W133A

Plasmid

#137655

Purpose

Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Article

Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

Insert

ppih (PPIH Human)

Tags

GST-(His)6-thrombin

Expression

Bacterial

Mutation

001-177-W133A

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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THI VP64 pALD4 T4

Plasmid

#161485

Purpose

CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T4_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (targeting T4 of pTHI11 promoter)

Expression

Yeast

Promoter

pALD4, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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THI VP64 pALD4 T5

Plasmid

#161486

Purpose

CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T5_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (targeting T5 of pTHI11 promoter)

Expression

Yeast

Promoter

pALD4, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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THI VP64 pGAP T2

Plasmid

#161489

Purpose

CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T2_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Targeting T2 in the THI11 promoter)

Expression

Yeast

Promoter

pGAP, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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THI VP64 pGAP T3

Plasmid

#161490

Purpose

CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T3_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Targeting T3 in the THI11 promoter)

Expression

Yeast

Promoter

pGAP, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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