We narrowed to 25,057 results for: Nes

1. 3xFLAG-dCas9/pTEF1p-CYC1t

   Plasmid

   #62190

   Purpose

   Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Yeast

   Mutation

   human codon-optimized, D10A, H840A

   Promoter

   TEF1 promoter

   Available Since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. p426-SNR52p-gRNA.csr-1.Y-SUP4t

   Plasmid

   #68060

   Purpose

   gRNA for csr-1 locus in N. crassa

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   gRNA for csr-1 locus
   

   Use

   CRISPR; N. crassa, fungi

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3-deltaBamHI-human MIZ1

   Plasmid

   #74167

   Purpose

   Mammalian expression of human MIZ1

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   MIZ1 (ZBTB17 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pBS NL4-3 envFS eGFP IN-D116A

   Plasmid

   #101333

   Purpose

   pBS NL4-3 envFS eGFP with mutation that disrupts IN catalytic activity

   Depositor

   Jeremy Luban

   Article

   McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

   Insert

   NL4-3 envFS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Integrase D116A

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD_C3006

   Plasmid

   #91096

   Purpose

   Module C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   GFP (+ BaeI site for GT donor cloning)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   FMV 34S

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

   Plasmid

   #79372

   Purpose

   Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-p65-hsf1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEE393

   Plasmid

   #149283

   Purpose

   T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3

   Depositor

   Daniel Voytas

   Article

   Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

   Insert

   p35S:TRV2_NbPDS3sgRNA_TrunFT:tNos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

8. pGL3-VEGFR2-225

   Plasmid

   #21306

   Depositor

   Donald Ingber

   Article

   Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

   Insert

   VEGFR2 promoter (KDR Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 29, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pMA-SpCas9-g7

   Plasmid

   #80790

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQTEV-PDAP1

    Plasmid

    #31586

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PDGFA associated protein 1 (PDAP1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRU54

    Plasmid

    #167680

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCVhygro-F-G9adSET

    Plasmid

    #41722

    Purpose

    Retroviral expression of human G9a mutant in mammalian cells

    Depositor

    Kai Ge

    Article

    Wang et al EMBO J. 2012 Nov 23. doi: 10.1038/emboj.

    Insert

    G9adset (EHMT2 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. dABE

    Plasmid

    #124447

    Purpose

    Expresses dead Cas9 (dCas9)-ABE7.10

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dABE7.10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSuper mouse Dicer1

    Plasmid

    #14779

    Depositor

    Tyler Jacks

    Article

    Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

    Insert

    Dicer shRNA (Dicer1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pRK5-mFzd9

    Plasmid

    #42261

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 9 (Fzd9 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pDEST14-SpyCatcher EQ

    Plasmid

    #35045

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyCatcher EQ
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Deletion of C-terminal till I113, and point mutat…

    Promoter

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

17. FKBP-AP180-GFP

    Plasmid

    #186577

    Purpose

    Expression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    AP180 (Snap91 Rat)
    

    Use

    Tags

    FKBP N-terminal GFP C-terminal

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pH6HTC_MANF-Halo

    Plasmid

    #175341

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    MANF (MANF Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS-H2B-EYFP

    Plasmid

    #53746

    Purpose

    contains fusion of human histone H2B to EYFP in pCS2+ vector.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    histone H2B (H2BC21 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    simian CMV IE94

    Available Since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pMOD_C3012

    Plasmid

    #91099

    Purpose

    Module C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    GFP (+ BaeI site for GT donor cloning)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    PvUbi1

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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