We narrowed to 19,041 results for: Met
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Plasmid#199666Purposeexpresses LOV-Turbo in the mammalian mitochondria, lentiviral vectorDepositorInsertLOV-Turbo
UseLentiviralTagsCOX4I1 mito targeting sequence and V5ExpressionMammalianPromoterTRE3GAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-E
Plasmid#78852PurposeIntroduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassetteDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsCas9-P2A-PuroExpressionMammalianAvailable SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ phiC31 integrase
Plasmid#216237PurposeFor the overexpression of phiC31 integraseDepositorInsertintegrase from phage φC31
UseSynthetic BiologyExpressionMammalianPromoterCMV IE94Available SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1-Full-Length-MePCE
Plasmid#113539PurposeProduces an N-terminal GST-tagged full length MePCE protein.DepositorAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
All_in_one_CRISPR/Cas9_LacZ
Plasmid#74293Purpose"All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screeningDepositorInsertsLacZ-alpha
Cas9
mCherry
UseCRISPRTagsHAExpressionBacterial and MammalianPromoterLac promoter, SV40, and T7 promoterAvailable SinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcmv3-IRG1-HA
Plasmid#198181PurposeMammalian expression of HA-tagged human ACOD1 (IRG1)DepositorInsertACOD1 (ACOD1 Human)
TagsHAExpressionMammalianMutationHA tag is added to C-terminal of target proteinPromoterCMVAvailable SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNvT-MHC::mCh
Plasmid#67943PurposeTransgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceIDepositorInsertmCherry
UseTransgenic reporter in the sea anemone, nematoste…PromoterMyosin Heavy Chain1 (MyHC1)Available SinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_DA2h
Plasmid#140554PurposeExpresses the 2nd generation high-affinity DA sensor GRAB_DA2h in neuronsDepositorHas ServiceAAV9InsertThe 2nd generation DA sensor GRAB_DA2h (high affinity)
UseAAVPromoterhSynAvailable SinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-fDIO-GFP-IRES-tTA
Plasmid#118026PurposeAAV vector for sparse and bright labeling in a cell-type specific mannerDepositorInsertTRE-fDIO-GFP-IRES-tTA
UseAAVMutationSee depositor comments belowPromoterTREAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro p53 shRNA 1
Plasmid#10671DepositorAvailable SinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GfaABC1D-GRAB_g5-HT3.0
Plasmid#208727PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0 in astrocytesDepositorInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0
UseAAVPromoterGfaABC1DAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule2-pCNF
Plasmid#85495PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
TagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657
Plasmid#212962PurposeLentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_non-targeting spacer
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianPromoterU6Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1.tR4-MbPyl
Plasmid#50832Purposebaculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cellsDepositorInsertstwo-copy Pyl tRNA cassette
MbPyl
two copy Pyl tRNA cassette
UseBaculoviralExpressionInsectPromoterCMV and U6 and H1Available SinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657
Plasmid#212963PurposeLentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_GFP targeting spacer
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianPromoterU6Available SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody
Plasmid#138130PurposeExpresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmCherry-anti-mouse-IgG(kappa)-nanobody
TagsGSTExpressionBacterialAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-NES-jRGECO1a-WPRE
Plasmid#216276PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1aDepositorInsertNES-jRGECO1a
UseAAV and Cre/LoxTags6XHIS and XpressPromoterhuman synapsin 1 (hSyn)Available SinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-HA-hYTHDC1
Plasmid#85167PurposeExpression of FLAG-HA-tagged human YTHDC1DepositorAvailable SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWPI-SLC7A11-HA
Plasmid#201643PurposeOver-expression of HA-tagged human SLC7A11DepositorAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCOLA-Gent-EM7-Erv1p-DsbC
Plasmid#202485PurposeExpresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutivelyDepositorInsertsulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)
ExpressionBacterialPromoterEM7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only