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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,901 results for: cloning

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Showing: 11521 - 11540 of 63901 results

  1. pAV105

    Plasmid
    #63195
    Purpose
    pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    63195-plasmid-map-sequence-id-104874

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  2. pAN19-3xFLAG-ccvTIR1-NOSt

    Plasmid
    #108546
    Purpose
    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator
    Depositor
    Keiko Torii
    Publication
    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d
    Insert
    TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    Use
    Cloning vector
    Tags
    3xFLAG
    Mutation
    Changed F79 to G
    Available since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108546-plasmid-map-sequence-id-210054

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  4. VanGlow-RR

    Plasmid
    #83343
    Purpose
    for Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.
    Depositor
    Cheng-Yu Lee
    Publication
    Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    mCherry::Renilla(nls)
    Expression
    Insect
    Available since
    Dec. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    83343-plasmid-map-sequence-id-158672

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  7. pMT-MCS-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80059
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80059-plasmid-map-sequence-id-152593

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  8. pAV106

    Plasmid
    #63196
    Purpose
    pRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    63196-plasmid-map-sequence-id-104896

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  9. pAV113

    Plasmid
    #63180
    Purpose
    pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63180-plasmid-map-sequence-id-104851

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  10. pMuLE ENTR 7SK-miR-30 L1-L4

    Plasmid
    #62180
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK RNA polymerase III promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    Gateway Cloning and RNAi
    Expression
    Mammalian
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5

    Plasmid
    #62127
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62127-plasmid-map-sequence-id-112700

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  12. pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3

    Plasmid
    #62131
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Type
    Empty backbone
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62131-plasmid-map-sequence-id-112709

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  13. PDG459 V3

    Plasmid
    #226958
    Purpose
    CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Publication
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226958-plasmid-map-sequence-id-487610

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  14. pHW2173

    Plasmid
    #255981
    Purpose
    N-terminal TAG-IN destination vector with SEC-LoxP for fusion tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
    Depositor
    Han Wang
    Insert
    AAG-SEC(pDD363 with LoxP)-GGT-rSapI-SpeI-SapI-ACG
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255981-plasmid-map-sequence-id-512522

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  15. pHW2174

    Plasmid
    #255982
    Purpose
    N-terminal TAG-IN destination vector with SEC-Lox2272 for fusion tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
    Depositor
    Han Wang
    Insert
    AAG-SEC(pDD382 with Lox2272)-GGT-rSapI-SpeI-SapI-ACG
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255982-plasmid-map-sequence-id-511513

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  16. pHW2175

    Plasmid
    #255983
    Purpose
    N-terminal TAG-IN destination vector with SEC-Lox511I for fusion tagging (slot 7), building TAG-IN donor plasmids via Gibson assembly, or cloning in a tag by SapTrap with a tag plasmid in slot 7.
    Depositor
    Han Wang
    Insert
    AAG-SEC(pDD364 with Lox511I)-GGT-rSapI-SpeI-SapI-ACG
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255983-plasmid-map-sequence-id-511495

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  19. pFTK092

    Plasmid
    #171364
    Purpose
    Vector part (LVL1) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    3'flanking region - pks17 Pc21g16000 (conidial pigment biosynthesis) P. rubens
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171364-plasmid-map-sequence-id-345500

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  20. pFTK091

    Plasmid
    #171363
    Purpose
    Vector part (LVL1) from the Fungal Modular Cloning ToolKit.
    Depositor
    Arnold Driessen
    Publication
    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021
    Insert
    5'flanking region - pks17 Pc21g16000 (conidial pigment biosynthesis) P. rubens
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    n/a
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171363-plasmid-map-sequence-id-345499

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Showing: 11521 - 11540 of 63901 results