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Plasmid#108838PurposeInsert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsPYL,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only
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pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA
Plasmid#108839PurposeInsert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsABIExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpA
Plasmid#108834PurposeInsert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFKBP,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA
Plasmid#108835PurposeInsert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2066_pCAG-PV1-NTD-ENTRY-L1-GID1-NLS-BGHpA
Plasmid#108844PurposeInsert protein fragment into MluI/BspEI to fuse to GID1/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
CHL1 wt-eGFP
Plasmid#117876Purposeprotein expressionDepositorInsertCHL1 wt-eGFP (AT5G40090 Mustard Weed)
UseTagsExpressionYeastMutationPromoterAOXAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
CHL1 T101D-eGFP
Plasmid#117880Purposeprotein expressionDepositorInsertCHL1T101D-eGFP (AT5G40090 Mustard Weed)
UseTagsExpressionYeastMutationT101DPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRESN2 H2B-GFP-uvr8at(2x)
Plasmid#44970DepositorUseTagsinternal GFPExpressionMammalianMutationtandem repeat of UVR8 (both have Methionine 1 del…PromoterpCMV IEAvailable sinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I
Plasmid#100922PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-LS-F138I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationF138IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-L255I
Plasmid#100964PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-LS-L255I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationL255IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only