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pET-21a_2xNLS_FnCpf1_MBP_protein_expression

Plasmid

#120801

Purpose

Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

2xNLS_FnCpf1

Use

CRISPR

Tags

MBP and NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/SPTBN1-FLT3

Plasmid

#168115

Purpose

Mammalian expression vector for the SPTBN1-FLT3 fusion gene identified in atypical chronic myeloid leukemia

Depositor

Nicholas Cross

Article

Grand et al Exp Hematol. 2007 Nov;35(11):1723-7. doi

Insert

SPTBN1-FLT3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPR01-FnCpf1

Plasmid

#176529

Purpose

Nuclease Cpf1 adapted for Aspergillus species

Depositor

Kusol Pootanakit

Article

Abdulrachman et al BMC Biotechnol. 2021 Feb 11;21(1):15. do

Insert

FnCpf1

Use

CRISPR; Fungal expression

Tags

Expression

Mutation

Promoter

TEF1

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJK526

Plasmid

#155387

Purpose

PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus
PphlF-TA4 in dCas12a oscillator crRNAs

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Promoter

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C1C2-GFP

Plasmid

#46044

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

Insert

nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV immediate early

Available since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only
pGEX2T merlin 340-590

Plasmid

#11633

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal half of merlin isoform 2.

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-FLEX-T-RG

Plasmid

#102368

Purpose

Express cre dependent TVA and SADB19 RG (biscistronic) as an AAV

Depositor

Troy Margrie

Article

Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

Insert

TVA E2A-RG

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX2T merlin 308-579

Plasmid

#11632

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal common domain of merlin.

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pFL-TK-AID3'UTR-MUT

Plasmid

#19987

Depositor

Nina Papavasiliou

Article

Teng et al Immunity. 2008 May . 28(5):621-9.

Insert

AID 3' UTR (Aicda Mouse)

Use

Tags

Expression

Mammalian

Mutation

Mutation

Promoter

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1_OCT4_S235A

Plasmid

#40639

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Use

Tags

GST, TEV Cleavage, TEV cleavage, Thrombin Cleavag…

Expression

Bacterial

Mutation

S235A

Promoter

tac

Available since

July 26, 2013

Availability

Academic Institutions and Nonprofits only