-
Plasmid#223380PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT09
Plasmid#223381PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.DepositorInsert2x35s-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT10
Plasmid#223382PurposeT-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-SpRY-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT01
Plasmid#223373PurposeT-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.DepositorInsertAtUBQ10-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT02
Plasmid#223374PurposeT-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpCas9-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT04
Plasmid#223376PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-SpCas9-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT06
Plasmid#223378PurposeT-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-SpCas9-ZmUbi-gRNA scaffold-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT07
Plasmid#223379PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.DepositorInsertAtUBQ10-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantMutationPromoterAvailable sinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-Lg-B
Plasmid#216960PurposeExpression of Large BiT-Linker-GvpBDepositorInsertLarge BiT-Linker-GvpB
UseTagsFLAG-Split Nanoluciferase LargeBiTExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET26b(modified)-B-Lg
Plasmid#216959PurposeExpression of GvpB-Linker-Large BiTDepositorInsertGvpB-Linker-Large BiT
UseTagsSplit Nanoluciferase LargeBiT-FLAGExpressionBacterialMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAf-CRISPR-ctfR1
Plasmid#207992PurposeCRISPR vector used with pAf-CRISPR-phoA (#207991) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavusDepositorInsertctfR1
UseCRISPRTagsExpressionMutationPromoterAspergillus flavus U6Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoG-NAD-NLS3x
Plasmid#193822PurposeCMV overexpression of ChemoG-NAD in the nucleus of mammalian cell linesDepositorInsertChemoG-NAD-NLS3x
UseTagsExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO-ChemoL-NAD-NLS3x
Plasmid#193828PurposeCMV overexpression of ChemoL-NAD in the nucleus of mammalian cell linesDepositorInsertChemoL-NAD-NLS3x
UseTagsExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO-[4xCox8]-ChemoL-NAD
Plasmid#193829PurposeCMV overexpression of ChemoL-NAD in the mitochondria of mammalian cell linesDepositorInsert[4xCox8]-ChemoL-NAD
UseTagsExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoB
Plasmid#193808PurposeCMV overexpression of ChemoB in mammalian cell linesDepositorInsertChemoB
UseTags10xHisExpressionMammalianMutationEBFP2: N39Y, V206K, T225R; HT7: E143R, E147R, L27…PromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoY
Plasmid#193810PurposeCMV overexpression of ChemoY in mammalian cell linesDepositorInsertChemoY
UseTags10xHisExpressionMammalianMutationVenus:A206K, T225R; HT7: E143R, E147R, L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJEP224-AAV-Basic-0.4αCaMKII-GFP-pA
Plasmid#62909PurposeAAV basic vector backbone designed to express GFP from a 0.4CaMKIIa promoter. It also contains a poly-Adenylation Signal (pA).DepositorInsertGreen Fluorescent protein (GFP)
UseAAVTagsExpressionMutationPromoter0.4αCaMKIIAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP221-AAV-RSV-GFP-pA
Plasmid#62919PurposeAAV vector backbone designed to express GFP from a RSV promoter. It also contains a poly-Adenylation Signal (pA)DepositorInsertGreen Fluorescent protein (GFP)
UseAAVTagsExpressionMutationPromoterRous Sarcoma Virus(RSV)Available sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_HSPA8-Halo
Plasmid#175325PurposeProduction of HaloTag fusion proteinDepositorInsertHSPA8 (HSPA8 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_xtHUR-Halo
Plasmid#175314PurposeProduction of HaloTag fusion proteinDepositorInsertHUR (elavl1 X. tropicalis (frog))
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only