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pCMV-TNT-CFP-STIM1-C227W

Plasmid

#68403

Purpose

Expresses a STIM1 gain-of-function mutant in mammalian cells

Depositor

Yubin Zhou

Article

Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

Insert

CFP-STIM1 (STIM1 Human)

Tags

Cyan Fluorescent Protein (CFP)

Expression

Mammalian

Mutation

Changed Cysteine 227 to Tryptophan; CFP was inser…

Promoter

CMV

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-TNT-CFP-STIM1-C227W

Plasmid

#68403

Purpose

Expresses a STIM1 gain-of-function mutant in mammalian cells

Depositor

Yubin Zhou

Article

Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.

Insert

CFP-STIM1 (STIM1 Human)

Tags

Cyan Fluorescent Protein (CFP)

Expression

Mammalian

Mutation

Changed Cysteine 227 to Tryptophan; CFP was inser…

Promoter

CMV

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
pLVX-Flag-CDK19-WT-IRES-ZsGreen

Plasmid

#68858

Purpose

Expresses Human CDK19 Wild-Type

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 19 (CDK19 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1α

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-CDK19-WT-IRES-ZsGreen

Plasmid

#68858

Purpose

Expresses Human CDK19 Wild-Type

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 19 (CDK19 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

EF1α

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-CDK19-W105M-IRES-ZsGreen

Plasmid

#68859

Purpose

Expresses Human CDK19 W105M Mutant

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 19 (CDK19 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

W105M

Promoter

EF1α

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-CDK19-W105M-IRES-ZsGreen

Plasmid

#68859

Purpose

Expresses Human CDK19 W105M Mutant

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 19 (CDK19 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

W105M

Promoter

EF1α

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PDGFB_DGCR2

Plasmid

#205893

Purpose

Express mEGFP-tagged fusion protein, PDGFB_DGCR2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PDGFB_DGCR2 (PDGFB, DGCR2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_PDGFB_DGCR2

Plasmid

#205893

Purpose

Express mEGFP-tagged fusion protein, PDGFB_DGCR2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

PDGFB_DGCR2 (PDGFB, DGCR2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OSKM

Plasmid

#80481

Purpose

piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OSKM

Plasmid

#80481

Purpose

piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pET_His6-SUMO-TEV-TtCsm6

Plasmid

#172486

Purpose

Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)

Tags

His-SUMO-TEV

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET_His6-SUMO-TEV-TtCsm6

Plasmid

#172486

Purpose

Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)

Tags

His-SUMO-TEV

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a_His-TEV-EiCsm6

Plasmid

#172487

Purpose

Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

Enterococcus italicus Csm6 (codon optimized)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a_His-TEV-EiCsm6

Plasmid

#172487

Purpose

Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

Enterococcus italicus Csm6 (codon optimized)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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peGFP NOVA2 op

Plasmid

#224354

Purpose

Express GFP-tagged human NOVA2 (codon optimized)

Depositor

Nicolas Charlet-Berguerand

Article

Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

Insert

NOVA2 (NOVA2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

codon optimized for expression in human cells

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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peGFP NOVA2 op

Plasmid

#224354

Purpose

Express GFP-tagged human NOVA2 (codon optimized)

Depositor

Nicolas Charlet-Berguerand

Article

Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

Insert

NOVA2 (NOVA2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

codon optimized for expression in human cells

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-7xTRE-3xeGFP

Plasmid

#191206

Purpose

GFP expression under the control of TRE promotor

Depositor

Janelia Viral Tools

Article

Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

Insert

3X GFP

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-7xTRE-3xeGFP

Plasmid

#191206

Purpose

GFP expression under the control of TRE promotor

Depositor

Janelia Viral Tools

Article

Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

Insert

3X GFP

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge