We narrowed to 16,202 results for: genome
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Plasmid#120423PurposeMultigene editing in the Escherichia coli genome using the CRISPR-Cas9 systemDepositorInsertCas9
UseCRISPRExpressionBacterialPromoteraraBADAvailable SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Rogi2-CMV-mRuby-bGH
Plasmid#179861PurposeTargets Rogi2 genomic region with HDR and expresses mRubyDepositorInsertmRuby
Available SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTTP1B
Plasmid#91723Purposeintegration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNADepositorTypeEmpty backboneUseIntegration-proficient plasmid vectors for mycoba…Available SinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLHDR01_mCherry_tCD19
Plasmid#123661PurposeConstruction of 1st donor for scarless genome editingDepositorTypeEmpty backboneUseMouse Targeting; Human targetingAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCpf1
Plasmid#122185PurposeBacterial genome editing plasmid with kanamycin resistance markerDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B7-deltaE4
Plasmid#122560PurposeModified block 7, with deletion in E4DepositorInsertAdenovirus 5 genomic region 30888-35938
UseSynthetic BiologyMutationAdenovirus sequences 32874-35461 replaced with Sp…Available SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRGEB33
Plasmid#158151PurposeConstruction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.DepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
SETDB1
Plasmid#25240PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETDB1, SETDB1_16 (SETDB1 Human)
Tags6xHis-TEV and MHHHHHHSSGRENLYFQGExpressionBacterialPromoterT7Available SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAd5-B1-deltaE1-MCS
Plasmid#122558PurposeModified block 1, with deletion in E1DepositorInsertAdenovirus 5 genomic region 1-3759
UseSynthetic BiologyMutationAdenovirus sequences 481-3530 replaced with multi…Available SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B6-deltaE3
Plasmid#122559PurposeModified block 6, with deletion in E3DepositorInsertAdenovirus 5 genomic region 27859-30803
UseSynthetic BiologyMutationAdenovirus sequences 27859-30803 replaced with Ba…Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable SinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
p33Lb
Plasmid#158156PurposeExpression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBTK1050
Plasmid#209540PurposeBTK Type 1 homology arm for Golden Gate assemblyDepositorInsertUpstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyAvailable SinceFeb. 9, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK1051
Plasmid#209541PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertRecombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
UseSynthetic BiologyAvailable SinceFeb. 2, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAH237
Plasmid#121146PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeastDepositorInsertsgRNA cassette
humanized Streptococcus pyogenes Cas9
UseCRISPRTagsFlagExpressionYeastPromoterS.pombe rrk1 and nmt41Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNH051
Plasmid#42157DepositorInsertGFP-RT-GFP
Available SinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAH243
Plasmid#121147PurposeExpression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeastDepositorInsertshumanized Streptococcus pyogenes Cas9
gRNA expression module
UseCRISPRTagsFlagExpressionYeastPromoterS.pombe rrk1 and nmt41Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRGEB34
Plasmid#158152PurposeConstruction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editingDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNH034
Plasmid#42150DepositorInsertRT-cassette
Available SinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHelper_O
Plasmid#173783PurposeThe 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.DepositorInsert186 Integrase
UseSynthetic BiologyExpressionBacterialMutationE59G - please see depositor commentsAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only