We narrowed to 15,752 results for: genome

1. pSLHDR01_mCherry_tCD19

   Plasmid

   #123661

   Purpose

   Construction of 1st donor for scarless genome editing

   Depositor

   Matthew Porteus

   Article

   Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

   Type

   Empty backbone

   Use

   Mouse Targeting; Human targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSU-araC-Cas9

   Plasmid

   #120423

   Purpose

   Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCpf1

   Plasmid

   #122185

   Purpose

   Bacterial genome editing plasmid with kanamycin resistance marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTTP1A

   Plasmid

   #91721

   Purpose

   integration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNA

   Depositor

   Graham Hatfull

   Article

   Pham et al Microbiology. 2007 Aug;153(Pt 8):2711-23

   Type

   Empty backbone

   Use

   Integration-proficient plasmid vectors for mycoba…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRGEB33

   Plasmid

   #158151

   Purpose

   Construction of inPTG-Cas9 plasmids expressing gRNA within an engineered intron for binary vector plant genome editing.

   Depositor

   Kabin Xie

   Article

   Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTTP1B

   Plasmid

   #91723

   Purpose

   integration-proficient plasmid vector for mycobacteria, contains the attP and int region from Tweety genomic DNA

   Depositor

   Graham Hatfull

   Article

   Pham et al Microbiology. 2007 Aug;153(Pt 8):2711-23

   Type

   Empty backbone

   Use

   Integration-proficient plasmid vectors for mycoba…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SETDB1

   Plasmid

   #25240

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SETDB1, SETDB1_16 (SETDB1 Human)
   

   Use

   Tags

   6xHis-TEV and MHHHHHHSSGRENLYFQG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pHA1

   Plasmid

   #85709

   Purpose

   This plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

   Depositor

   Martin Messerle, Ulrich Koszinowski

   Article

   Adler et al J Virol. 2000 Aug;74(15):6964-74.

   Type

   Empty backbone

   Use

   Cre/Lox; Herpesvirus cloning

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDropVn

   Plasmid

   #184873

   Purpose

   Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pNH051

    Plasmid

    #42157

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    GFP-RT-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAd5-B1-deltaE1-MCS

    Plasmid

    #122558

    Purpose

    Modified block 1, with deletion in E1

    Depositor

    Fred Bunz

    Article

    Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

    Insert

    Adenovirus 5 genomic region 1-3759
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Adenovirus sequences 481-3530 replaced with multi…

    Promoter

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p33Lb

    Plasmid

    #158156

    Purpose

    Expression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing.

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available Since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAd5-B6-deltaE3

    Plasmid

    #122559

    Purpose

    Modified block 6, with deletion in E3

    Depositor

    Fred Bunz

    Article

    Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

    Insert

    Adenovirus 5 genomic region 27859-30803
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Adenovirus sequences 27859-30803 replaced with Ba…

    Promoter

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAH237

    Plasmid

    #121146

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    gRNA cassette
    humanized Streptococcus pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1 and nmt41

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRGEB34

    Plasmid

    #158152

    Purpose

    Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing

    Depositor

    Kabin Xie

    Article

    Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNH034

    Plasmid

    #42150

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    RT-cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHelper_O

    Plasmid

    #173783

    Purpose

    The 186 helper plasmid necessary for genomic integration of the pIT5_KO plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    186 Integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    E59G - please see depositor comments

    Promoter

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHelper_W

    Plasmid

    #173784

    Purpose

    The Wphi helper plasmid necessary for genomic integration of the pIT5_KW plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    Wphi Integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHelper_D

    Plasmid

    #173781

    Purpose

    The D145 helper plasmid necessary for genomic integration of the pIT5_KD plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    D145 Integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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