We narrowed to 150,311 results for: ins

1. pUb-G76V-GFP-IRES-DsRed

   Plasmid

   #225503

   Purpose

   Expression of Ub-G76V-GFP along dsRED from one mRNA

   Depositor

   David Rubinsztein

   Article

   Yu et al Hum Mol Genet. 2024 May 22:ddae085. doi:

   Insert

   Ub-G76V-GFP
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Ubiquitin fused to N-terminus of GFP. Glycine 76 …

   Promoter

   CMV promoter

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-SdrG-B2-ELP-HIS-ybbR

   Plasmid

   #169134

   Purpose

   E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Tags

   3xELP linker, 6x Histag, and ybbr tag

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SdrG-B1-ELP-HIS-ybbR

   Plasmid

   #169133

   Purpose

   E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Tags

   3xELP linker, 6x Histag, and ybbr tag

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGS_N_Strep

   Plasmid

   #101651

   Purpose

   Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, KpnI linker, Twin-Strep tag
   

   Tags

   Twin-Strep tag

   Expression

   Insect

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGS_C_Strep

   Plasmid

   #101652

   Purpose

   Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

   Depositor

   Monika Golas, Bjoern Sander

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, MCS linker, Twin-Strep tag
   

   Tags

   Twin-Strep tag

   Expression

   Insect

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. SBC009876

   Plasmid

   #226283

   Purpose

   Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

   Depositor

   Nigel Scrutton

   Article

   Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

   Inserts

   Coniferyl alcohol acyltransferase
   Eugenol synthase 1
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. LLPS1-iLID::EGFP::FTH1 (pBS1042)

   Plasmid

   #185289

   Purpose

   For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   iLID::eGFP::FTH1
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. ApoE3_mCh-SspB (pBS1144)

   Plasmid

   #185326

   Purpose

   For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE3
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ApoE2_mCh-SspB (pBS1142)

   Plasmid

   #185324

   Purpose

   For the mammalian expression of the human protein ApoE2 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   ApoE2
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. hTln1-R1R2-17b

    Plasmid

    #191427

    Purpose

    Expresses a variant of the human TLN1 R1R2 domains containing a 17AA insert into R2 (exon 17b) in bacteria

    Depositor

    Ben Goult

    Article

    Gallego-Paez et al J Cell Biol. 2023 May 1;222(5):e20220901

    Insert

    Human Talin1 R1R2 domains (TLN1 Human)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    contains 17AA insertion from inclusion of exon 17b

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

    Plasmid

    #185299

    Purpose

    Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_shuffle_3
    

    Expression

    Mammalian

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. DHN_VrDHN1a_mCh-SspB (pBS1074)

    Plasmid

    #185298

    Purpose

    Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k3_26
    

    Expression

    Mammalian

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ApoE3_D125I_47-300_mCh-SspB (pBS1071)

    Plasmid

    #185294

    Purpose

    For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_D125I_47-300
    

    Expression

    Mammalian

    Mutation

    D125I

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. ApoE3_D125I_mCh-SspB (pBS1145)

    Plasmid

    #185327

    Purpose

    For the mammalian expression of the human protein ApoE3_D125I attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE3_D125I
    

    Expression

    Mammalian

    Mutation

    D125I

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. ApoE4_mCh-SspB (pBS1143)

    Plasmid

    #185325

    Purpose

    For the mammalian expression of the human protein ApoE4 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    ApoE4
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pvLEA22mer_mutant_3_mCh-SspB (pBS1080)

    Plasmid

    #185303

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_mutant_3
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PvLEA4_repeats_k5_1_mCh-SspB (pBS1079)

    Plasmid

    #185302

    Purpose

    For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    PvLEA4_repeats_k5_1
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pvLEA22mer_mutant_10_mCh-SspB (pBS1078)

    Plasmid

    #185301

    Purpose

    For the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    pvLEA22mer_mutant_10
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAHS2_PARRC_98-130_mCh-SspB (pBS1077)

    Plasmid

    #185300

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2_PARRC_98-130
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. DSUP_RAMVA_1-208_mCh-SspB (pBS1073)

    Plasmid

    #185296

    Purpose

    For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DSUP_RAMVA_1-208
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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