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pDONR223_SMAD4_p.P215S

Plasmid

#82829

Purpose

Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

P215S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD4_p.D493N

Plasmid

#82788

Purpose

Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

D493N

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD4_p.V492F

Plasmid

#82785

Purpose

Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

V492F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD4_p.G386V

Plasmid

#82772

Purpose

Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

G386V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAB31_WT_V5

Plasmid

#82993

Purpose

Gateway Donor vector containing RAB31, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RAB31 (RAB31 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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NEK2 gRNA (BRDN0001145653)

Plasmid

#76215

Purpose

3rd generation lentiviral gRNA plasmid targeting human NEK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NEK2 (NEK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_p.E4K

Plasmid

#82860

Purpose

Gateway Donor vector containing RBM10, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Mutation

E4K; E849D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_p.Y580F

Plasmid

#82834

Purpose

Gateway Donor vector containing RBM10 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Mutation

Y580F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMiR-RECK-3'UTR-miR-7-mut

Plasmid

#53692

Purpose

Luciferase reporter assay for RECK 3'UTR that has point mutations on miR-7 binding site

Depositor

Edward Chan

Article

Jung et al J Biol Chem. 2012 Aug 24;287(35):29261-7

Insert

RECK-3'UTR (RECK Human)

Use

Luciferase

Mutation

nucleotide position 171-174 are mutated to GAAG

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pMIR-Report-Luc-KLF4-K5S-Mt344A

Plasmid

#34601

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Luciferase

Expression

Mammalian

Mutation

The KLF4 K5S region was mutated at the miR-344 bi…

Promoter

CMV

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only
pCS2_hsPum2_hsAdar2

Plasmid

#250539

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of human Pum2 fused to the catalytic domain of human Adar2

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

hsPum2_hsAdar2 (ADARB1, PUM1 Human)

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pDonor MAFA-P2A-tdTomato

Plasmid

#246654

Purpose

Donor plasmid for Crispr/Cas9 gene editing of the human MAFA locus

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

P2A-tdTomato flanked by MAFA homology regions (MAFA Synthetic, Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLJC2-HK2 (D209A, D657A)-3xFLAG

Plasmid

#239244

Purpose

HK2 lentiviral overexpression vector

Depositor

Jason Cantor

Article

Huggler et al Nat Metab. 2026 Jan 16. doi: 10.1038/s42

Insert

HK2 (HK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert contains the set of silent mutations descr…

Promoter

CMV

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-EF1α-DIO-DSE-mCherry-PSE-shRNA-Numb

Plasmid

#250238

Purpose

Cre-dependent AAV expressing mCherry and an shRNA targeting Numb under EF1α promoter for selective Numb knockdown

Depositor

Won Do Heo

Article

Shin et al Exp Mol Med. 2025 Aug;57(8):1818-1836. d

Insert

Numb (Numb Mouse)

Use

AAV, Cre/Lox, Mouse Targeting, and RNAi

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-DIO-optoFGFR1-HA

Plasmid

#250235

Purpose

Cre-dependent AAV expressing light-activatable FGFR1 (optoFGFR1-HA) for temporally precise control of neuronal FGFR1 signaling

Depositor

Won Do Heo

Article

Shin et al Exp Mol Med. 2025 Aug;57(8):1818-1836. d

Insert

FGFR1 (FGFR1 Human)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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pX462-ABE8e-nSaCas9

Plasmid

#237462

Purpose

Transfection-based all-in-one base editor plasmid that expresses both adenine base editor (ABE8e-nSaCas9) and single guide RNAs (sgRNA) with BpiI cloning sites to clone sgRNA.

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Kumari et al Nat Commun. 2026 Feb 3;17(1):1218. doi:

Insert

3xFlag_ABE8e_SaCas9 -6xNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A

Promoter

chicken beta-actin promoter & U6

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pDonor AVIL-P2A-iCre

Plasmid

#246652

Purpose

Donor plasmid for Crispr/Cas9 gene editing of the human AVIL locus

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

P2A-iCre flanked by AVIL homology regions (AVIL Synthetic, Human)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pME-FastFucci-P2A-H2A-tdiRFP (JDW 1399)

Plasmid

#242557

Purpose

Gateway middle entry clone containing mammalian FastFUCCI reporter followed by H2A tdiRFP reporter; For visualizing cell cycle state. tdiRFP reporter labels all cells regardless of cell cycle state

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Use

Gateway subcloning

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-gRNA22-3

Plasmid

#248550

Purpose

A CRISPR-based technology to study chromosome-specific aneuploidy by targeting human centromeres

Depositor

Teresa Davoli

Article

Bosco et al Cell. 2023 Apr 27;186(9):1985-2001.e19.

Insert

gRNA22-3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-shDrp1-mCherry-CAAX

Plasmid

#228739

Purpose

Membrane-tethered mCherry shRNA targeting Drp1

Depositor

Cagla Eroglu

Article

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Drp1 (Dnm1l, Dnm1l Rat, Mouse)

Expression

Mammalian

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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