We narrowed to 24,104 results for: CRISPR

1. PB-U6

   Plasmid

   #120358

   Purpose

   piggyBac transposon vector for sgRNA expression.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET302-6His-dCas9-Halo

   Plasmid

   #72269

   Purpose

   Encodes a Halo-labeled fusion of nuclease deficient Cas9

   Depositor

   Timothée Lionnet

   Article

   Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

   Insert

   His6-dCas9-Halo
   

   Available Since

   Jan. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA_expression_vector

   Plasmid

   #210212

   Purpose

   An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

   Depositor

   Albert Jeltsch

   Article

   Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

   Type

   Empty backbone

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGDestTol2LC-5sgRNA

   Plasmid

   #64243

   Purpose

   destination vector for five U6x:sgRNA cassettes

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2_B-act_dCas9-Vp64-T2A-Citrine

   Plasmid

   #200248

   Purpose

   Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

   Depositor

   Tatjana Sauka-Spengler

   Article

   Pelea et al bioRxiv 2023.05.08.539738

   Insert

   dCas9-Vp64-T2A-Citrine
   

   Use

   Zebrafish expression

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6_N7-sgRNA2

   Plasmid

   #190664

   Purpose

   Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   N7 sgRNA2
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYZ038

   Plasmid

   #200227

   Purpose

   universal template to build tRNA-gRNA array for multiplex editing CREEPY

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

   Insert

   tRNA-gRNA scaffold
   

   Use

   Synthetic Biology

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEJS2699-CMV-Nme2Cas9-E932R

   Plasmid

   #228720

   Purpose

   Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2Cas9-E932R nuclease
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS2696-CMV-Nme2SmuCas9-D56R

   Plasmid

   #228717

   Purpose

   Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2SmuCas9-D56R nuclease
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D56R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS2697-CMV-Nme2SmuCas9-E932R

    Plasmid

    #228718

    Purpose

    Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2SmuCas9-E932R nuclease
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS2698-CMV-Nme2Cas9-D56R

    Plasmid

    #228719

    Purpose

    Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Nme2Cas9-D56R nuclease
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D56R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-Simple-gRNA backbone

    Plasmid

    #51306

    Purpose

    gRNA expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Type

    Empty backbone

    Use

    CRISPR; Xenopus expression

    Promoter

    T7

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMJ920

    Plasmid

    #42234

    Depositor

    Jennifer Doudna

    Article

    Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    GFP, HA epitope, and NLS (nuclear localization si…

    Expression

    Mammalian

    Mutation

    codon-optimized synthetic DNA sequence

    Promoter

    CMV

    Available Since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cas9m4-VP64

    Plasmid

    #47319

    Purpose

    Cas9m4 Activator

    Depositor

    George Church

    Article

    Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

    Insert

    Cas9m4-VP64
    

    Use

    CRISPR

    Available Since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pG1

    Plasmid

    #162602

    Purpose

    Edit Ade2 gene in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9 with RPL25 Intron
    

    Expression

    Yeast

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. BCJJ345

    Plasmid

    #117504

    Purpose

    YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtYAO

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. p-dCas9-LSD1-Hygro

    Plasmid

    #104406

    Purpose

    transient expression of dCas9-LSD1 fusion protein

    Depositor

    Stephan Beck

    Insert

    LSD1 (KDM1A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    IRATp970A0364D (Source Bioscience) has a P405H ch…

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFD3-NS

    Plasmid

    #149545

    Purpose

    Expression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGGDestTol2LC-4sgRNA

    Plasmid

    #64242

    Purpose

    destination vector for four U6x:sgRNA cassettes

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGH224_sgRNA_2xMS2_Puro

    Plasmid

    #85413

    Purpose

    hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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