We narrowed to 16,144 results for: LEA
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Plasmid#112859PurposePlasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFPDepositorInsertmCherry-apoB-EGFP
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
cr3Vn
Plasmid#184861Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination systemDepositorInsertCas9, gam, bet, exo
TagsCas9 ssrA degradation tagExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_7653_v2
Plasmid#126855Purpose7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffoldDepositorInsertII_CS3_7653_v2
UseSynthetic BiologyAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
AMPKA2
Plasmid#36194PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertAMPKA2 (PRKAA2 Human)
TagsHis tag with thrombin cleavageExpressionBacterialMutationcontains KINASE DOMAIN onlyAvailable SinceAug. 8, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-N1-mt-ro1GFP
Plasmid#82407PurposeExpression of redox sensitive GFP with a mitochondrial targeting sequence in mammalian cellsDepositorInsertro1GFP
Tagsleader sequence of the E1α subunit of pyruvate de…ExpressionMammalianMutationC48S/F64L/T65S/S147C/Q204CPromoterCMVAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046979
Plasmid#145611PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Wild-type CoV-2 ORF8
Plasmid#159933PurposeProtein overexpression in E.coliDepositorInsertCoV-2 ORF8 18-121 (ORF8 CoV-2)
Tags6x Histidine, TEV cleavableExpressionBacterialMutationNonePromoterT7Available SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
mVenus-hCOL1A1
Plasmid#140111PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
TagsmVenusExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable SinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-UBC9-K14R-A129K
Plasmid#228948Purposeexpress a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)DepositorInsertUBC9 (UBE2I Human)
TagsHis tag and thrombine cleavage site : MGHHHHHHSSG…ExpressionBacterialMutationchanged Lysine 14 to Arginine, changed Alanine 12…PromoterT7-lacAvailable SinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-G97 IRES EGFP
Plasmid#136620PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertSNAP-Tag-LAMA-G97-NLS (IRES EGFP)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-H_NES_C1
Plasmid#85061PurposeIn vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)DepositorInsertNES
ExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046920
Plasmid#145717PurposeBacterial Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF HaloTag-SYB2
Plasmid#197282PurposeEncodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.DepositorAvailable SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoC
Plasmid#193809PurposeCMV overexpression of ChemoC in mammalian cell linesDepositorInsertChemoC
Tags10xHisExpressionMammalianMutationmCerulean3:T225R; HT7: E143R, E147R, L271EPromoterCMVAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (1-575)
Plasmid#1318DepositorInsertHsp104 (1-575) (HSP104 Budding Yeast)
Tags6X His + TEV cleavageExpressionBacterialMutationaa1-575 of Hsp104Available SinceJan. 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag-NLS-Smad2
Plasmid#14931DepositorInsertNLS-Smad2 (SMAD2 Human)
TagsFlagExpressionMammalianMutationfull-length Smad2 is fused to a nuclear-localizat…Available SinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nDsRedFL-intron
Plasmid#177804PurposeAn intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.DepositorInsertintron
ExpressionMammalianAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nuc-ABKAR
Plasmid#61511PurposeExpression of nuclear AMPK biosensorDepositorInsertAMPK Biosensor
TagsNLS (see comments)ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
mini-donor - NKX2.5-nEGFP
Plasmid#209287Purposethis mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locusDepositorInsertEGFP-nls
UseKnockin donor vectorAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-wtSpCas9-H840A
Plasmid#80453PurposeSpCas9 mammalian expression vectorDepositorInsertSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianPromoterCBhAvailable SinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only