We narrowed to 15,989 results for: LEA

1. V1-MESA-45F-M-tTA

   Plasmid

   #84500

   Purpose

   MESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and tTA

   Depositor

   Joshua Leonard

   Article

   Schwarz et al Nat Chem Biol. 2016 Dec 12. doi: 10.1038

   Insert

   V1-MESA-45F-M-tTA
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

   Plasmid

   #31477

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   SFFV HA NLS Y2 T2A mTagBFP
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Mutation

   F13Y + S111Y on I-AniI = Y2 "reo" (res…

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDEST14-SpyCatcher002 EQ

   Plasmid

   #102830

   Purpose

   Bacterial expression of unreactive version of SpyCatcher002

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002 EQ
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   E77Q mutation prevents isopeptide bond formation

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJOG536

   Plasmid

   #105423

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   Nuclear targeting factor (RanGAP1(WPP)-GFP-BLRP)
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGem-nHyPer2:sHyper2

   Plasmid

   #84737

   Purpose

   Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   HyPer2 targeted to nucleus
   

   Tags

   NLS-SV40

   Expression

   Plant

   Promoter

   pFMV/p35S

   Available Since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGBW-m4046754

   Plasmid

   #145647

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pGBW-m4046747

   Plasmid

   #149249

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 3C-like proteinase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   May 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. dFP-mini

   Plasmid

   #120543

   Purpose

   Expresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.

   Depositor

   Brian Chow

   Article

   Sheehan et al Biochemistry. 2018 Nov 28. doi: 10.1021/

   Insert

   de novo fluorescence-activating protein mini (dFP-mini)
   

   Tags

   6xHis - TEV cleavage

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SP6-hCas9-Ce-mRNA

   Plasmid

   #47911

   Purpose

   In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.

   Depositor

   Paul Sternberg

   Article

   Chiu et al Genetics. 2013 Aug 26.

   Insert

   hCas9 with C. elegans 5' and 3' UTRs
   

   Use

   CRISPR

   Promoter

   SP6

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGBW-m4046733

    Plasmid

    #145665

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. 1137=Hsp70Bb-Cas9[dsRed]

    Plasmid

    #149424

    Purpose

    The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Promoter

    Dmel Hsp70Bb

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmScarlet-i_NES_C1

    Plasmid

    #85062

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCB11

    Plasmid

    #201111

    Purpose

    pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.

    Depositor

    Mary Dunlop

    Article

    Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:

    Inserts

    CcaR
    tetA(C)
    sfGFP
    

    Expression

    Bacterial

    Mutation

    C-to-T transition at pos. no. 1049 of tetA(C) lea…

    Promoter

    J23100 and PcpcG2-172

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBW-m4046795

    Plasmid

    #145598

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGBW-m4046910

    Plasmid

    #145672

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pJK506

    Plasmid

    #155382

    Purpose

    dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBW-m4046740

    Plasmid

    #145588

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 endoRNAse

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. mPapaya-Nup50-N-10

    Plasmid

    #56348

    Purpose

    Localization: Nuclear Pore, Excitation: 530, Emission: 541

    Depositor

    Michael Davidson

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Insert

    Nup50 (NUP50 Human)
    

    Tags

    mPapaya

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pISH-NQO1

    Plasmid

    #136766

    Purpose

    Template to synthesize riboprobes for in situ hybridization (ISH) for Nqo1

    Depositor

    Peter Mombaerts

    Article

    Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

    Insert

    Nqo1 (Nqo1 Mouse)
    

    Use

    In situ probe

    Mutation

    ISH Probe insert, V184A- Please see depositor co…

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGBW-m4046916

    Plasmid

    #145627

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge