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prNFL-L269P-EGFP

Plasmid

#132601

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E L269P mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

806T>C, 807G>C; Leu269Pro

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-E397K-EGFP

Plasmid

#132603

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E E397K mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

1189G>A; Glu397Lys

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-P441L-EGFP

Plasmid

#132604

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P441L mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Mutation

1322C>T, 1323A>G; Pro441Leu

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6_Ptac_BmHps_BmPhi_BsMdh

Plasmid

#129103

Purpose

Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

Depositor

Eleftherios Terry Papoutsakis

Article

Whitaker et al Metab Eng. 2017 Jan;39:49-59. doi: 10.10

Insert

BsMDH BmHPS BmPHI

Expression

Bacterial

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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ptagBFP-TetR-NLS-VP16

Plasmid

#103835

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-TetR-NLS-VP16 (tetR )

Expression

Mammalian

Promoter

SV2

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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alpha7-nAChR(scramble control)

Plasmid

#86649

Purpose

expression of control shRNA

Depositor

Jaime Modiano

Article

Oloris et al Toxicol Appl Pharmacol. 2010 Feb 1;242(3

Insert

CHRNA7 (control)

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6C-(928-1020)-NES-mut

Plasmid

#42027

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6C (TNRC6C Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa928-1020, L943A, L947A, M950A, F952A

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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DVA_AG

Plasmid

#66042

Purpose

MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [A:LacZa:G]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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phNFL-N98S

Plasmid

#132592

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

NEFL (NEFL Human)

Expression

Mammalian

Mutation

293A>G; Asn98Ser

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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miR-141 reporter

Plasmid

#67632

Purpose

To quantify miR-141 function

Depositor

Judy Lieberman

Article

Le et al J Clin Invest. 2014 Dec;124(12):5109-28.

Insert

2 binding sites for miR-141

Use

Luciferase

Promoter

SV40

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
GST-CLCb

Plasmid

#47404

Depositor

Peter McPherson

Article

Legendre-Guillemin et al J Biol Chem. 2005 Feb 18;280(7):6101-8.

Insert

CLCb

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pCuGFP-SpAtg17(416)

Plasmid

#122080

Purpose

Expressing S. pombe GFP-Atg17

Depositor

Daniel Klionsky

Article

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG17 (atg17 Fission Yeast)

Tags

GFP

Expression

Yeast

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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DVA_DH

Plasmid

#66049

Purpose

MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:H]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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DVA_EB

Plasmid

#66050

Purpose

MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-hum-LFA

Plasmid

#20051

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

S492A

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01(ΔL2)

Plasmid

#80050

Purpose

Expression of EPN-01 mutated in ALIX binding site.

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01(deltaYP)

Tags

Myc

Expression

Mammalian

Mutation

YP37AA

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Prp2-pRSETA

Plasmid

#61042

Purpose

Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.

Depositor

Nils Walter

Article

Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

Insert

Prp2

Tags

Histidine Tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-EPN-01*(ΔL1)

Plasmid

#80042

Purpose

Expression of PTAP L domain mutant EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

pCMV-EPN-01*(ΔL2)

Tags

Myc

Expression

Mammalian

Mutation

PTAP sequence in p6 mutated to Ala

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01*(ΔL2)

Plasmid

#80043

Purpose

Expression of YPxL L domain mutantd EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*(L33R)

Tags

Myc

Expression

Mammalian

Mutation

YP37 in p6 mutated to AA

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-hum-LYS

Plasmid

#20047

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

F489Y

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only