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pFB2D

Plasmid

#195286

Purpose

Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection

Depositor

Scott Lowe

Article

Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

Insert

DTR EGFP 2A Puro

Expression

Mammalian

Promoter

EFS

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_WT

Plasmid

#98657

Purpose

Expresses 6xHis-tagged hnRNPA2 AA 190-341

Depositor

Nicolas Fawzi

Article

Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMIA10.7

Plasmid

#214451

Purpose

BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Inserts

Tet-On 3G
PuroR
TRE3G CopGFP

Use

Bxbi donor

Tags

PuroR, T2A, and Tet-On 3G

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(SMARCA2(46))-PGKpuro2ABFP-W

Plasmid

#200465

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA2(46) (SMARCA2 Human)

Use

Lentiviral

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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shCTNNB1 # 2

Plasmid

#42544

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pMAL-XPG

Plasmid

#39215

Depositor

Aziz Sancar

Article

Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

Insert

XPG (ERCC5 Human)

Tags

MBP

Expression

Bacterial

Mutation

Note that XPG starts at amino acid 5

Promoter

Ptac

Available Since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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shCTNNB1 # 1

Plasmid

#42543

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-GRAM1b

Plasmid

#211494

Purpose

Expression of EGFP-tagged GRAM domain of GRAMD1b (EGFP-GRAM1b) in mammalian cells

Depositor

Yasunori Saheki

Article

Naito et al Elife. 2019 Nov 14;8. pii: 51401. doi: 1

Insert

GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pKL038 Lenti U6 dCas12a gRNA Puro mCherry

Plasmid

#195546

Purpose

dCas12a gRNA expression backbone

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti U6- empty cassette_Direct repeat_puro_mcherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBX_Sec61b_smGFP_Myc

Plasmid

#236092

Purpose

Constitutively encodes Sec61b-smGFP-Myc (sfGFP-based spaghetti monster fluorescent protein embedding Myc epitope tags), for targeting the endoplasmic reticulum.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

Sec61b-smGFP-Myc (SEC61B Human, Synthetic)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas9-GFP

Plasmid

#181906

Purpose

Fluorescently tagged dead Cas9

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9

Use

CRISPR

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 LRP6-eGFP

Plasmid

#180143

Purpose

to make movies of LRP6 vesicles

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Expression

Mammalian

Promoter

SP6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-hPMLIII

Plasmid

#181903

Purpose

Fluorescently tagged PMLIII

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

hPMLIII (PML Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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BcLOV4 fusion tools screening plasmid #1

Plasmid

#174511

Purpose

Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

[BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT5_TRE-ARID1A-FLAG_PGK-H2B-RFP

Plasmid

#203965

Purpose

Dox inducible FLAG tag ARID1a in a sleeping beauty transposon construct

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

ARID1A (Arid1a Mouse)

Use

Sleeping beauty transposon

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Grhl3

Plasmid

#172869

Purpose

Mammalian expression vector with myc-tagged mouse Grhl3 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Grhl3 (Grhl3 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

Plasmid

#170273

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2

Expression

Mammalian

Mutation

See Comments

Promoter

CMVd1

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-pCALM1-sFLEx-HA-SpCas9-miniU6-sgRNAShank3

Plasmid

#213969

Purpose

AAV vector for encoding SpCas9 driven by pCALM1 promoter targeting Shank3 locus in the presence of Cre recombinase

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pES001 MBP-GSK3β-HA-His

Plasmid

#154010

Purpose

Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GSK3B (GSK3B Human)

Tags

HA tag, His6, and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA6.2EmGFP-DAXX

Plasmid

#169726

Purpose

Mammalian expression construct of GFP tagged full length DAXX

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

DAXX (DAXX Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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