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We narrowed to 29,279 results for: Tat

Showing: 11701 - 11720 of 29279 results
  1. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid
    #129042
    Purpose
    inactivated control (targeted DNA demethylation_human_TSDR), expression of dCas9-hudTET1CD-T2A-mCherry sgRNA2targeting human TSDR
    Depositor
    Insert
    dCas9-hudTET1CD, SgRNA2 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid
    #129030
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR
    Depositor
    Insert
    dCas9-huTET1CD, SgRNA2 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
  3. pGL3-Basic-ZC3H12D_PromoterMUT

    Plasmid
    #153067
    Purpose
    ZC3H12D promoter region carrying mutations in BHLHE40 binding sites, cloned upstream of luciferase reporter
    Depositor
    Insert
    ZC3H12D promoter region mutated in BHLHE40 binding sites (ZC3H12D Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Mutagenesis to disrupt 3 BHLHE4040 binding sites.…
    Promoter
    None
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. Desmoplakin I no force control (F40-based)

    Plasmid
    #118726
    Purpose
    The no force control for the F40-based human desmoplakin I tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Insert
    human Desmoplakin I (1-1952)-[YPet(short)-F40-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-F40-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1952
    Promoter
    CMV
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pb-NES-ZapCV5 (cpV143)

    Plasmid
    #112061
    Purpose
    Genetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Low affinity to free zinc (Kd ~ 300nM, n = 0.55) due to mutation of the zinc binding Cys residues to His.
    Depositor
    Insert
    NES-ZapCV5
    Tags
    Nuclear Export Signal (NES) derived from HIV-1
    Expression
    Mammalian
    Mutation
    ECFP gene is truncated 36 bp at 3' end. Venu…
    Promoter
    CBA
    Available Since
    July 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pFUSEss-CHIg-mM-Arg210Ala_M18

    Plasmid
    #91734
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg210Ala (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Arg210 changed to Ala (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pFUSEss-CHIg-mM-Asn209Arg_M18

    Plasmid
    #91740
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asn209Arg (EU numbering).
    Depositor
    Insert
    immunoglobulin heavy constant mu (Ighm Mouse)
    Expression
    Mammalian
    Mutation
    Asn209 changed to Arg (EU numbering)
    Promoter
    hEF1-HTLV prom
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 11701 - 11720 of 29279 results