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POC1554

Plasmid

#238471

Purpose

pMOBC360_VR: Vector Right (VR) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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POC1525

Plasmid

#226496

Purpose

pMOBC360_VM: Vector Middle (VM) of the set (Chloramphenicol R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1520

Plasmid

#221581

Purpose

pMOBK360_VR: Vector Right (VR) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1518

Plasmid

#221579

Purpose

pMOBK360_VL: Vector Left (VL) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1519

Plasmid

#221580

Purpose

pMOBK360_VM: Vector Middle (VM) of the set (Kanamycin R) to be methylated by the M.Osp807II BsaI-associated switch methylase. Used for the assembly using the methylation protection approach

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pEYD1A

Plasmid

#127759

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach, MG RNA aptamer in divergent orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEYT1A

Plasmid

#127760

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach, MG RNA aptamer in tandem orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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AAEL017341 TK R

Plasmid

#115412

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

tachykinin-like peptides receptor 99D

Expression

Mammalian

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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TM SE T206A

Plasmid

#79664

Purpose

More dimeric SE attached to Triple Mutant GEVI from Piao et al 2015

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

Triple Mutant SE T206A

Expression

Mammalian

Mutation

T206A

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-C

Plasmid

#60979

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic cerulean

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

Cerulean

Use

Zebrafish

Promoter

Bactin2

Available Since

Feb. 3, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-O

Plasmid

#60982

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes cytoplasmic mKO

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

mKO

Use

Zebrafish

Promoter

Bactin2

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pEYmRD3A

Plasmid

#127756

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach,RFP Divergent Orientation

Use

Synthetic Biology

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

Plasmid

#174665

Purpose

Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

Depositor

Eli Bar

Inserts

LUC2
mCherry:T2A:Hygro

Use

Lentiviral and Luciferase

Tags

mCherry and hygromycin dual reporter gene linked …

Expression

Mammalian

Promoter

Human cytomegalovirus immediate early enhancer/pr…

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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Ubiquitin WT

Plasmid

#12647

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

Ubiquitin - wild type (UBC Human)

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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pHH0103_PCMT1_PCMT

Plasmid

#109932

Purpose

Protein expression and purification of PCMT1_PCMT

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PCMT1_PCMT

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-hM3D(Gq)-mCherry

Plasmid

#50460

Purpose

Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter

Depositor

Bryan Roth

Insert

hM3D(Gq)-mCherry (CHRM3 Human)

Use

AAV

Tags

mCherry

Mutation

See supplemental documents for DREADD mutations

Promoter

EF1a

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAQ_14-3-3

Plasmid

#109943

Purpose

Protein expression and purification of YWHAQ_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAQ_14-3-3

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC

Plasmid

#52985

Purpose

split Venus attached to MCP and PCP respectively

Depositor

Robert Singer

Article

Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

Insert

nls-ha-MCP-VenusN-nls-ha-PCP-VenusC

Use

Lentiviral

Promoter

ubc

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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polySIM

Plasmid

#221068

Purpose

Expression of polySIM protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4

Tags

His and MBP

Expression

Bacterial

Promoter

unknown

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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polySUMO3

Plasmid

#221067

Purpose

Expression of polySUMO3 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of SUMO3 each separated by (GGS)4 (SUMO3 Human)

Tags

His and MBP

Expression

Bacterial

Promoter

tac

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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