Addgene

We narrowed to 8,890 results for: Pol;

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pTS38.3

Plasmid

#106266

Purpose

Polycistronic expression of ekivR, esyn1, luc using P2A

Depositor

Vera Meyer

Article

Schuetze et al Microb Cell Fact. 2017 Sep 25;16(1):162.

Insert

ekivR-P2A-esyn1-P2A-luc

Use

Luciferase and Synthetic Biology

Tags

Expression

Mutation

Promoter

Tet-On

Available since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-T

Plasmid

#87522

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-A

Plasmid

#87523

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-C

Plasmid

#87524

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALEN-STAR-G

Plasmid

#87525

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STARVP64-T

Plasmid

#87530

Purpose

Destination vector for TALE-VP64 construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (activator)

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STARVP64-A

Plasmid

#87531

Purpose

Destination vector for TALE-VP64 construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (activator)

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STARVP64-C

Plasmid

#87532

Purpose

Destination vector for TALE-VP64 construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (activator)

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STARVP64-G

Plasmid

#87533

Purpose

Destination vector for TALE-VP64 construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (activator)

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STARVP160-T

Plasmid

#87534

Purpose

Destination vector for TALE-VP160 construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (activator)

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STARVP160-A

Plasmid

#87535

Purpose

Destination vector for TALE-VP160 construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (activator)

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STARVP160-C

Plasmid

#87536

Purpose

Destination vector for TALE-VP160 construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (activator)

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pTALE-STARVP160-G

Plasmid

#87537

Purpose

Destination vector for TALE-VP160 construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN; Tale-tf (activator)

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBK-CMV Pig12

Plasmid

#16506

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig12 (PTGES Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
V96 ELP

Plasmid

#68392

Purpose

Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V96 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-RPA194

Plasmid

#17660

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RPA194 (Polr1a Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
Tubp-Gal80 in pCaSpeR4

Plasmid

#17748

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

GAL80 (GAL80 Budding Yeast)

Use

Tags

SV40 polyA and Tubulin 1alpha promoter

Expression

Insect

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

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pMEG_YLCas9_empty

Plasmid

#117826

Purpose

CRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolytica

Depositor

Michael Sauer

Article

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_hCas9_YlCyc1TT

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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V72 ELP

Plasmid

#68938

Purpose

Elastin-like Polypeptide (VPGxG) with 72 repeats containing Valine guest residue

Depositor

Andrew MacKay

Article

Dhandhukia et al Biomacromolecules. 2013 Apr 8;14(4):976-

Insert

V72 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-8x-gRNA-eGFP

Plasmid

#60718

Purpose

eGFP reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_MFE1

Plasmid

#84612

Purpose

Introduces DSB at MFE1 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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Tubp-Gal4 in pCaSpeR4

Plasmid

#17747

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

GAL4 (GAL4 Budding Yeast)

Use

Tags

Tubulin 1alpha promoter and hsp70 PolyA

Expression

Insect

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pCRISPRyl_D17

Plasmid

#84611

Purpose

Introduces DSB at D17 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_A08

Plasmid

#84610

Purpose

Introduces DSB at A08 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMtBS

Plasmid

#32226

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

MCV Small t antigen

Use

Tags

Expression

Mammalian

Mutation

Splice site was silently mutated, and a restricti…

Promoter

Available since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pCRISPRyl_XPR2

Plasmid

#84609

Purpose

Introduces DSB at XPR2 locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4 Pig1

Plasmid

#16494

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig1 (LGALS7 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pCRISPRyl_AXP

Plasmid

#84608

Purpose

Introduces DSB at AXP locus in Y. lipolytica

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB103-Citrine

Plasmid

#79137

Purpose

Binary vectors for expression of Citrine fluorescent protein in M. polymorpha

Depositor

Yutaka Kodama

Article

Tsuboyama et al Plant Cell Physiol. 2014 Jan;55(1):229-3

Insert

Citrine

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

MpEF1a promoter

Available since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pST50Trc1-HISNDHFR

Plasmid

#63940

Purpose

Position 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

Depositor

Song Tan

Article

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Use

Tags

6xHis; TEV cleavable

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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