We narrowed to 5,507 results for: SPI

1. p5E_hs_Glast (JDW 1182)

   Plasmid

   #224483

   Purpose

   Gateway compatible 5' entry clone containing the Human GLAST promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   GLAST promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5E-EF1a (JDW 1164)

   Plasmid

   #224473

   Purpose

   Gateway compatible 5' entry clone containing the human EF1a promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   EF1a promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5E-hs_Cldn5 (JDW 1120)

   Plasmid

   #224481

   Purpose

   Gateway compatible 5' entry clone containing the Human Claudin 5 promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Claudin-5 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pME AmCyan (JDW 1151)

   Plasmid

   #224485

   Purpose

   Gateway compatible middle entry clone containing AmCyan cytosolic blue flourescent reporter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   AmCyan
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME V5 mScarlet-I (JDW 1323)

   Plasmid

   #224487

   Purpose

   Gateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   V5-mScarlet-I
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-mCerulean3-TUBB1A (JDW 1367)

   Plasmid

   #224503

   Purpose

   Gateway compatible middle entry clone containing mCerulean3 fused to TUBB1A for visualizing tubulin in cells

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   mCerulean3-TUBB1A
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-LexA-mODC (no LexOp) (JDW 937)

   Plasmid

   #224504

   Purpose

   Gateway compatible middle entry clone containing a destabilized LexA transactivator

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   LexA-mODC
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pME-myc-BirA (JDW 1210)

   Plasmid

   #224509

   Purpose

   Gateway compatible middle entry clone containing a Myc tagged BirA

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Myc-BirA
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-Myc-BioID2-MCS1 (JDW 1095)

   Plasmid

   #224510

   Purpose

   Gateway compatible middle entry clone containing a Myc-tagged BIOID2 construct

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Myc-BioID2
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p3E-H2A_mCherry_SV40pA (JDW 967)

    Plasmid

    #224528

    Purpose

    A Gateway compatible 3' entry clone containing an H2A mCherry fusion followed by SV40 late polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    H2A-mCherry-SV40pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-V5-mClover3-pA (JDW 1318)

    Plasmid

    #224530

    Purpose

    A Gateway compatible 3' entry clone containing a V5 tagged mClover3 followed by a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    V5-mClover3-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3E-IRES-mTagBFP2-pA (JDW 1361)

    Plasmid

    #224535

    Purpose

    A Gateway compatible 3' entry clone containing an IRES-FLAG-NLS-mTagBFP2-pA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    IRES-FLAG-NLS-mTagBFP2-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-IRES-(n2)StayGold(c4)-pA (JDW 1364)

    Plasmid

    #224537

    Purpose

    A Gateway compatible 3' entry clone containing IRES-(n2)StayGold(c4)-pA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    IRES-(n2)StayGold(c4)-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-Lifeact- mScarlet-HA (JDW 1247)

    Plasmid

    #224538

    Purpose

    A Gateway compatible middle entry clone containing Lifeact-mScarlet-I (far red f-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Lifeact-mScarlet-HA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pME H2B mCerulean (JDW 1150)

    Plasmid

    #224543

    Purpose

    A Gateway compatible middle entry clone containing a histone H2B fusion to mCerulean to label the nucleus

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    H2B-mCerulean
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPRISM-QF2-he1.1:YFP

    Plasmid

    #184819

    Purpose

    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    QF2-pA-he1.1:YFP
    

    Use

    CRISPR; Backbone for cloning of homology arms (fo…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPRISM-QF2-he1.1:YFP-rln3a-HA

    Plasmid

    #184820

    Purpose

    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    rln3a-HA
    

    Use

    CRISPR; For integration of qf2 by homology-direct…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TOPO-nmba

    Plasmid

    #184821

    Purpose

    Used to synthesize RNA probe for in situ hybridization for nmba

    Depositor

    Marnie Halpern

    Article

    Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

    Insert

    nmba (nmba Zebrafish)
    

    Use

    Template for synthesis of nmba probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. FAS promoter luciferase

    Plasmid

    #8890

    Depositor

    Bruce Spiegelman

    Article

    Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

    Insert

    Fatty Acid Synthetase Promoter (Fasn Rat, unknown - rat or mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pME Actin Vhh mCherry (JDW 1240)

    Plasmid

    #224499

    Purpose

    Gateway compatible middle entry clone containing an Actin nanobody fused to mCherry (For visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Actin-Vhh-mCherry
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge